caa a22 4500 467901260 CHVBK 20180406152818.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0080-7 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0080-7 Transformation of Actinidia eriantha [Elektronische Daten] A potential species for functional genomics studies in Actinidia [Tianchi Wang, Yidong Ran, Ross Atkinson, Andrew Gleave, Dan Cohen] Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (β-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants. Springer-Verlag, 2005 Actinidia eriantha nationallicence Agrobacterium -mediated transformation nationallicence Functional genomics nationallicence Kiwifruit nationallicence Wang Tianchi The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut Ran Yidong The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut Atkinson Ross The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut Gleave Andrew The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut Cohen Dan The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 425-431 0721-7714 25:5<425 2006 25 299 https://doi.org/10.1007/s00299-005-0080-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0080-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Tianchi The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut NATIONALLICENCE 700 1- Ran Yidong The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut NATIONALLICENCE 700 1- Atkinson Ross The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut NATIONALLICENCE 700 1- Gleave Andrew The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut NATIONALLICENCE 700 1- Cohen Dan The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, New Zealand aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 425-431 0721-7714 25:5<425 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer