caa a22 4500 467901317 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0088-z doi (NATIONALLICENCE)springer-10.1007/s00299-005-0088-z In vitro germination and transient GFP expression of American chestnut ( Castanea dentata ) pollen [Elektronische Daten] [Danilo Fernando, Javonna Richards, Julie Kikkert] The development of the male reproductive structures of American chestnut (Castanea dentata) is described to advance our understanding of its reproductive behavior. This information has been vital in the development of a strategy to collect pollen grains from male catkins suitable for in vitro germination and transformation experiments. Cutting male catkins into small segments and rolling them over a culture plate resulted in evenly dispersed and large amounts of pollen with minimal unwanted accessory floral parts. To optimize pollen viability, the effect of various storage conditions on in vitro germination was examined. Our results showed that initial storage at 4°C for 2 weeks significantly increased percent germination as compared to freshly collected pollen and those stored directly at −20°C or −80°C. This also means that for long-term storage of American chestnut pollen, the catkins should first be kept at 4°C for a couple of weeks and then at −80°C. The use of pollen grains with high viability is necessary for the transformation of American chestnut pollen. To optimize pollen transformation via particle bombardment, the effects of target distance, target pressure, and pollen developmental stage were examined. Statistical analysis showed that bombardment of ungerminated pollen at 1,100psi resulted in the highest percent transient GFP expression (4.1%). Springer-Verlag, 2005 American chestnut nationallicence Castanea dentata nationallicence Pollen nationallicence Transformation nationallicence Viability nationallicence Fernando Danilo Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, 241 Illick Hall, 1 Forestry Drive, 13210, Syracuse, NY, USA aut Richards Javonna Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, 241 Illick Hall, 1 Forestry Drive, 13210, Syracuse, NY, USA aut Kikkert Julie Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 450-456 0721-7714 25:5<450 2006 25 299 https://doi.org/10.1007/s00299-005-0088-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0088-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernando Danilo Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, 241 Illick Hall, 1 Forestry Drive, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Richards Javonna Department of Environmental and Forest Biology, State University of New York College of Environmental Science and Forestry, 241 Illick Hall, 1 Forestry Drive, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Kikkert Julie Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 450-456 0721-7714 25:5<450 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer