caa a22 4500 467901325 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0077-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0077-2 Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice ( Oryza sativa L.) [Elektronische Daten] [Jong-In Park, Hirokazu Hakozaki, Makoto Endo, Yoshinobu Takada, Hitoshi Ito, Masanori Uchida, Tomihiro Okabe, Masao Watanabe] In our previous cDNA microarray analysis, we identified 53 mature anther-specific genes, whose function was unknown, in rice. We reanalyzed these genes from the viewpoint of the specific amino acid motif. Out of 53 genes, three genes, Os-26, Os-32, and Os-169 (renamed as OsSCP1, OsSCP2, and OsSCP3), encoded cysteine-rich motif (Cys-X3-Cys-X13-Cys-X3-Cys), indicating that they were novel small cysteine-rich proteins. From the search of specific elements in promoter regions, several pollen-specific elements were found. In order to determine whether three promoters were functional in pollen or not, the gene constructs with promoter regions fused to the β-glucuronidase gene were transformed into tobacco. Histochemical analysis showed that these promoters were active in the mature pollen grains and pollen tubes. Furthermore, OsSCP1 and OsSCP3 formed a multigene family tandemly in the rice genome. From the results, OsSCPs might have important roles in mature pollen development and pollen tube growth. Springer-Verlag, 2005 Gene family nationallicence Mature pollen-specific genes nationallicence Oryza sativa L nationallicence Promoter analysis nationallicence Small cysteine-rich protein nationallicence GUS : β-glucuronidase nationallicence OsSCPs : Oryza sativa small cysteine-rich proteins nationallicence RT-PCR : reverse transcription - polymerase chain reaction nationallicence Park Jong-In Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Hakozaki Hirokazu Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Endo Makoto Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Takada Yoshinobu Division of Natural Science, Osaka Kyoiku University, 582-8582, Kashiwara, Japan aut Ito Hitoshi Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Uchida Masanori Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Okabe Tomihiro Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut Watanabe Masao Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, 980-8577, Sendai, Japan aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 466-474 0721-7714 25:5<466 2006 25 299 https://doi.org/10.1007/s00299-005-0077-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0077-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Jong-In Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Hakozaki Hirokazu Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Endo Makoto Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Takada Yoshinobu Division of Natural Science, Osaka Kyoiku University, 582-8582, Kashiwara, Japan aut NATIONALLICENCE 700 1- Ito Hitoshi Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Uchida Masanori Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Okabe Tomihiro Faculty of Agriculture, Iwate University, 020-8550, Morioka, Japan aut NATIONALLICENCE 700 1- Watanabe Masao Laboratory of Plant Reproductive Genetics, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, 980-8577, Sendai, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 466-474 0721-7714 25:5<466 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer