caa a22 4500 467901333 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0079-0 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0079-0 Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens [Elektronische Daten] [Sung Cho, Quoc Hoang, Yoon Kim, Hyun Shin, Sung Ok, Jung Bae, Jeong Shin] Physcomitrella patens is a model plant for studying gene function using a knockout strategy. To establish a proteome database for P. patens, we resolved over 1,500 soluble proteins from gametophore and protonema tissues by two-dimensional electrophoresis (2-DE) and obtained peptide mass fingerprints (PMFs) by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Using expressed sequence tags (ESTs), we were able to predict the identities of 90 protein spots. Most of these were related to energy or primary metabolism. Comparative proteome analysis was used to identify proteins specific for each of the tissue types. One of these was a metallothionein type-2 (PpMT2) protein that was highly upregulated in gametophore tissue. PpMT2 was induced in both the gametophore and protonema following culture on solid media and in response to various abiotic stresses such as copper, cadmium, cold, indole-3-acetic acid, and ethylene. We suggest that PpMT2 is not only involved in metal binding and detoxification, but also in many biological aspects as a metal messenger or a protein with additional functions. Springer-Verlag, 2005 2-DE: Two-dimensional electrophoresis nationallicence PMFs: Peptide mass fingerprints nationallicence MALDI-TOF-MS: Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry nationallicence EST: Expressed sequence tag nationallicence MT: Metallothionein nationallicence Cho Sung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Hoang Quoc School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Kim Yoon School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Shin Hyun School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Ok Sung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Bae Jung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Shin Jeong School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 475-488 0721-7714 25:5<475 2006 25 299 https://doi.org/10.1007/s00299-005-0079-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0079-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cho Sung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Hoang Quoc School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Yoon School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Shin Hyun School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Ok Sung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Bae Jung School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Shin Jeong School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 475-488 0721-7714 25:5<475 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer