caa a22 4500 467901341 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s00299-005-0069-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0069-2 Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants [Elektronische Daten] [Dora Li, Jennifer O'Leary, Yan Huang, Norman Huner, Anthony Jevnikar, Shengwu Ma] A DNA construct containing the cholera toxin B subunit (CTB) gene genetically fused to a nucleotide sequence encoding three copies of tandemly repeated diabetes-associated autoantigen, the B chain of human insulin, was produced and transferred into low-nicotine tobaccos by Agrobacterium. Integration of the fusion gene into the plant genome was confirmed by polymerase chain reaction (PCR). The results of immunoblot analysis verified the synthesis and assembly of the fusion protein into pentamers in transgenic tobacco. GM1-ELISA showed that the plant-derived fusion protein retained GM1-ganglioside receptor binding specificity. The fusion protein accounted for 0.11% of the total leaf protein. The production of transgenic plants expressing CTB-InsB3 offers a new opportunity to test plant-based oral antigen therapy against autoimmune diabetes by inducing oral tolerance. Springer-Verlag, 2005 Autoimmune diabetes nationallicence Cholera toxin B subunit (CTB) nationallicence Oral tolerance nationallicence Transgenic plant expression system nationallicence The B chain of human insulin nationallicence Li Dora Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut O'Leary Jennifer Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut Huang Yan Plantigen Inc., 700 Collip Circle, N6G 4X8, London, Ontario, Canada aut Huner Norman Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut Jevnikar Anthony Plantigen Inc., 700 Collip Circle, N6G 4X8, London, Ontario, Canada aut Ma Shengwu Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 417-424 0721-7714 25:5<417 2006 25 299 https://doi.org/10.1007/s00299-005-0069-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0069-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Dora Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut NATIONALLICENCE 700 1- O'Leary Jennifer Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut NATIONALLICENCE 700 1- Huang Yan Plantigen Inc., 700 Collip Circle, N6G 4X8, London, Ontario, Canada aut NATIONALLICENCE 700 1- Huner Norman Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut NATIONALLICENCE 700 1- Jevnikar Anthony Plantigen Inc., 700 Collip Circle, N6G 4X8, London, Ontario, Canada aut NATIONALLICENCE 700 1- Ma Shengwu Department of Biology, University of Western Ontario, N6A 5B7, London, Ontario, Canada aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/5(2006-05-01), 417-424 0721-7714 25:5<417 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer