caa a22 4500 46790135X CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0068-3 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0068-3 Modification of erucic acid content in Indian mustard ( Brassica juncea ) by up-regulation and down-regulation of the Brassica juncea FAT TY ACID ELONGATION1 ( BjFAE1 ) gene [Elektronische Daten] [S. Kanrar, J. Venkateswari, P. Dureja, P. Kirti, V. Chopra] In Brassicas, the Fatty Acid Elongation1 (FAE1) gene product, a 3-ketoacyl-CoA synthase, is the first in a 4-enzyme complex involved in the synthesis of erucic acid from oleic acid. The FAE1 homologue from Brassica juncea cv. Pusa Bold was cloned in a binary vector both in sense and antisense orientations under the control of the CaMV35S promoter. The recombinant binary vectors were used to transform B. juncea cv. RLM 198 via Agrobacterium tumefaciens. The presence of the transgene was confirmed by polymerase chain reaction and Southern hybridization. Northern and western analyses showed the expression of the gene and protein, respectively, in the transgenic plants. Analyses of the fatty acid profile of the seed oil from homozygous T4 generation seeds revealed that over-expression of the FAE1 gene caused a 36% increase in the percent of erucic acid (37-49% compared to 36% in untransformed control). The down-regulation of FAE1 caused an 86% decrease in the percent of erucic acid to as low as 5% in the seed oil of transgenic plants. Thus, it is clearly possible to alter erucic acid content of mustard by altering the expression level of the FAE 1 gene. Springer-Verlag, 2005 Brassica juncea nationallicence Indian mustard nationallicence Fatty acid elongation 1 nationallicence Erucic acid nationallicence Very long chain fatty acids nationallicence FAE1 : Fatty acid elongation 1 nationallicence BAP : Benzyl amino purine nationallicence IAA : Indoleacetic acid nationallicence CaMV : Cauliflower mosaic virus nationallicence npt II : Neomycin phosphotransferase II gene nationallicence PCR : Polymerase chain reaction nationallicence VLCFA : Very long chain fatty acids nationallicence Kanrar S. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Venkateswari J. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Dureja P. Division of Agricultural Chemicals, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Kirti P. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Chopra V. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 148-155 0721-7714 25:2<148 2006 25 299 https://doi.org/10.1007/s00299-005-0068-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0068-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanrar S. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Venkateswari J. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Dureja P. Division of Agricultural Chemicals, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Kirti P. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Chopra V. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 148-155 0721-7714 25:2<148 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer