caa a22 4500 467901368 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0050-0 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0050-0 Genetic and biochemical analysis of Hypericum perforatum L. plants regenerated after cryopreservation [Elektronische Daten] [Martina Urbanová, Ján Košuth, Eva Čellárová] Shoot-tips from in vitro cultured Hypericum perforatum L. genotypes were subjected to assessments of developmental competence, genetic stability, and biosynthetic ability to identify critical points during cryopreservation. Survival rate, chromosome number stability, alteration in VNTR sequences and hypericin content were evaluated, in plants after pre-culture, and two subsequent cryogenic steps (cryoprotection and cooling) and those recovered from cryopreserved meristems. Pre-culture and cryoprotection treatments, did not reveal any significant differences, in these studied characteristics. Genetic stability was assessed by chromosome counts and analysis of variability in the VNTR sequences. No changes in chromosome number were detected in comparison with the untreated control but minor alterations were revealed in non-coding sequences. The content of hypericin after the recovery of cryopreserved meristems remained comparable with the unfrozen control. The controlled rate freezing technique used for cryopreservation was relevant for restoration of genetic and biochemical stability in Hypericum perforatum L. shoot-tips. Springer-Verlag, 2005 Chromosome number nationallicence Hypericin content nationallicence Survival rate nationallicence VNTR sequences nationallicence ABA : abscisic acid nationallicence BA : 6-benzylaminopurine nationallicence DMSO : dimethyl sulfoxide nationallicence LN : liquid nitrogen nationallicence SSR : simple sequence repeats nationallicence VNTR : variable number of tandem repeats nationallicence Urbanová Martina Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut Košuth Ján Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut Čellárová Eva Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 140-147 0721-7714 25:2<140 2006 25 299 https://doi.org/10.1007/s00299-005-0050-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0050-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Urbanová Martina Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut NATIONALLICENCE 700 1- Košuth Ján Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut NATIONALLICENCE 700 1- Čellárová Eva Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 04154, Košice, Slovakia aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 140-147 0721-7714 25:2<140 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer