caa a22 4500 467901376 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0008-2 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0008-2 Reverse transcriptase domain sequences from Mungbean ( Vigna radiata ) LTR retrotransposons: Sequence characterization and phylogenetic analysis [Elektronische Daten] [Anupam Dixit, Kyung-Ho Ma, Jae-Woong Yu, Eun-Gi Cho, Yong-Jin Park] The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences possessed stop codons or frame-shifts or both. The RT sequences corresponding to both the groups exhibit significant levels of heterogeneity. Presence of mungbean copia and gypsy RT sequences in other papilionoid legumes of the same (Phaseoleae) and different lineages (Loteae, Trifoleae, Cicereae) indicates existence of these elements prior to the radiation of papilionoid legumes and also supports the recent interpretations of close relationship between Phaseoleae and Loteae tribes of Papilionoideae subfamily. On the other hand significant homologies of some mungbean copia as well as gypsy RT sequences with those of unrelated plant species suggest their origin from different plant lineages and also that heterogeneous population of related elements were already existed throughout (even before the divergence of monocot and dicot) the evolution of these genera from their common ancestor. Springer-Verlag, 2005 Mungbean nationallicence Ty1-copia and Ty3-gypsy retrotransposons nationallicence Sequence heterogeneity nationallicence Reverse transcriptase nationallicence Genome nationallicence LTR : Long terminal repeat nationallicence PCR : Polymerase chain reaction nationallicence RT : Reverse transcriptase nationallicence Dixit Anupam Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut Ma Kyung-Ho Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut Yu Jae-Woong Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut Cho Eun-Gi Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut Park Yong-Jin Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 100-111 0721-7714 25:2<100 2006 25 299 https://doi.org/10.1007/s00299-005-0008-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0008-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dixit Anupam Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut NATIONALLICENCE 700 1- Ma Kyung-Ho Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut NATIONALLICENCE 700 1- Yu Jae-Woong Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut NATIONALLICENCE 700 1- Cho Eun-Gi Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut NATIONALLICENCE 700 1- Park Yong-Jin Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, 441-707, Suwon, Kyunggi, Republic of Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 100-111 0721-7714 25:2<100 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer