caa a22 4500 467901392 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0041-1 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0041-1 Exogenous and endogenous growth regulators on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis [Elektronische Daten] [V. Menéndez, N. Villacorta, M. Revilla, V. Gotor, P. Bernard, H. Fernández] This work showed for the first time the relationship between the effect of exogenous auxins and gibberellins on apogamy in Dryopteris affinis (Lowe) Fraser-Jenkins sp. affinis and its endogenous contents during early apogamic events. The addition of NAA (0.53 and 5.37μM) or GA3 2.8μM to an MS solid medium significantly increased apogamous sporophyte formation. BA induced brown callus that regenerated sporophytes in a hormone-free medium. The endogenous contents of GA1, GA3, GA4, GA7, GA9 and IAA were determined by GC-MS in gametophytes cultured on MS solid medium, before and during early stages of apogamous embryo development. The accumulation of both GA9 and IAA before embryo development was evident as high levels of GA4 in the earliest analysed stage of embryo development and high levels of GA3 in elongating shoots were found. The role of gibberellins on apogamy was also supported by data showing a decrease in the percentage of gametophytes developing embryos because of the addition of flurprimidol to the culture medium. Springer-Verlag, 2005 Apogamy nationallicence Dryopteris affinis sp. affinis nationallicence Fern nationallicence Gametophyte nationallicence BA : 6-Benzyladenine nationallicence GA3 : Gibberellic acid nationallicence DW : Dry weight nationallicence GC-MS : Gas chromatography-mass spectrometry nationallicence IAA : Indole-3-acetic acid nationallicence m / z : Mass to charge ratio nationallicence MS medium : Murashige and Skoog mineral medium nationallicence NAA : Naphtaleneacetic acid nationallicence Menéndez V. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut Villacorta N. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut Revilla M. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut Gotor V. Dpt. Química Orgánica e Inorgánica, c/ Julián Clavería, s/n, 33071, Oviedo, Spain aut Bernard P. Dpt. Química Orgánica e Inorgánica, c/ Julián Clavería, s/n, 33071, Oviedo, Spain aut Fernández H. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 85-91 0721-7714 25:2<85 2006 25 299 https://doi.org/10.1007/s00299-005-0041-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0041-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menéndez V. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Villacorta N. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Revilla M. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Gotor V. Dpt. Química Orgánica e Inorgánica, c/ Julián Clavería, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Bernard P. Dpt. Química Orgánica e Inorgánica, c/ Julián Clavería, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 700 1- Fernández H. Area de Fisiología Vegetal, Dpt. B.O.S., c/ Catedrático R Uría, s/n, 33071, Oviedo, Spain aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 85-91 0721-7714 25:2<85 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer