caa a22 4500 467901406 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0036-y doi (NATIONALLICENCE)springer-10.1007/s00299-005-0036-y Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola [Elektronische Daten] [Ri-He Peng, Quan-Hong Yao, Ai-Sheng Xiong, Zong-Ming Cheng, Yi Li] Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzyme can be glycosylated in the seeds of transgenic plants and retain a high stability during storage. These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems. Springer-Verlag, 2005 Agrobacterium- mediated transformation nationallicence Aspergillus phytase nationallicence Brassica napus nationallicence Codon modification nationallicence Endoplasmic reticulum retention signal nationallicence GUS : β-glucuronidase nationallicence MPHY2 : modified phytase gene nationallicence PR-S : pathogen-related protein S nationallicence SAR : scaffold attachment regions nationallicence Peng Ri-He Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut Yao Quan-Hong Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut Xiong Ai-Sheng Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut Cheng Zong-Ming Department of Plant Sciences, University of Tennessee, 37996-4500, Knoxville, TN, USA aut Li Yi Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 124-132 0721-7714 25:2<124 2006 25 299 https://doi.org/10.1007/s00299-005-0036-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0036-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Ri-He Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut NATIONALLICENCE 700 1- Yao Quan-Hong Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut NATIONALLICENCE 700 1- Xiong Ai-Sheng Shanghai Key Laboratory of Agricultural Genetics and Breeding, Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, People's Republic China aut NATIONALLICENCE 700 1- Cheng Zong-Ming Department of Plant Sciences, University of Tennessee, 37996-4500, Knoxville, TN, USA aut NATIONALLICENCE 700 1- Li Yi Department of Plant Science, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 124-132 0721-7714 25:2<124 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer