caa a22 4500 467901414 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0055-8 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0055-8 A novel isoform of ATPase c subunit from pearl millet that is differentially regulated in response to salinity and calcium [Elektronische Daten] [Wricha Tyagi, SnehLata Singla-Pareek, Suresh Nair, M. Reddy, S. Sopory] Vacuolar ATPases help in maintaining the pH of the vacuoles and thereby play a crucial role in the functioning of vacuolar sodium-proton antiporter. Though the various subunits that make V1 and V0 sector have been reported in plants their regulation is not understood completely. We have cloned three different isoforms of vacuolar ATPase subunit c (VHA-c) from Pennisetum glaucum with homologies among themselves varying from 38% to ∼73% at the nucleic acid level. Using real-time PCR approach we have shown that the three isoforms are regulated in a tissue-specific manner under salinity stress. While isoform III is constitutively expressed in roots and shoots and does not respond to stress, isoform I is upregulated under stress. Isoform II is expressed mainly in roots; however, under salinity stress its expression is downregulated in roots and upregulated in shoots. Tissue specific expression under salinity stress of isoform II was also seen after exogenous application of calcium. This study for the first time shows the presence of three isoforms of PgVHA-c and their differential regulation during plant development, and also under abiotic stress. Springer-Verlag, 2005 Abiotic stress nationallicence Differential regulation nationallicence Pennisetum nationallicence Real-time PCR nationallicence Vacuolar ATPase nationallicence Tyagi Wricha International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Singla-Pareek SnehLata International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Nair Suresh International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Reddy M. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Sopory S. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 156-163 0721-7714 25:2<156 2006 25 299 https://doi.org/10.1007/s00299-005-0055-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0055-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tyagi Wricha International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Singla-Pareek SnehLata International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Nair Suresh International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Reddy M. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Sopory S. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 156-163 0721-7714 25:2<156 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer