caa a22 4500 467901422 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0046-9 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0046-9 Differential mRNA stability to endogenous ribonucleases of the coding region and 3′ untranslated regions of wheat ( Triticum aestivum L.) manganese superoxide dismutase genes [Elektronische Daten] [Kwang-Hyun Baek, Daniel Skinner] The sequences of the 3′ untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3′ UTR variants and the coding region were measured following exposure to endogenous nucleases. The degradation rates of the 3′ UTR variants for 15min were not significantly different, meaning the degradation rates of the 3′ UTR variants were not directly related to the thermostabilities. However, the degradation rate of the coding region was significantly faster than those of the 3' UTR variants. Further investigation revealed the coding region seemed to have specific sites for degradation, indicating a possibility of increasing MnSOD expression by the degradation site alteration. Springer-Verlag, 2005 Manganese superoxide dismutase nationallicence Multigene family nationallicence Quantitative real time PCR nationallicence RNA secondary structure nationallicence RNA stability nationallicence Wheat nationallicence cDNA : complementary DNA nationallicence MnSOD : mitochondrial manganese-superoxide dismutase nationallicence qRT-PCR : quantitative real time PCR nationallicence 3′ UTR : 3′ untranslated region nationallicence Baek Kwang-Hyun Department of Crop and Soil Sciences, Washington State University, 210 Johnson Hall, 99164-6420, Pullman, WA, USA aut Skinner Daniel USDA/ARS, Department of Crop and Soil Science, Washington State University, 209 Johnson Hall, 99164-6420, Pullman, WA, USA aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 133-139 0721-7714 25:2<133 2006 25 299 https://doi.org/10.1007/s00299-005-0046-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0046-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baek Kwang-Hyun Department of Crop and Soil Sciences, Washington State University, 210 Johnson Hall, 99164-6420, Pullman, WA, USA aut NATIONALLICENCE 700 1- Skinner Daniel USDA/ARS, Department of Crop and Soil Science, Washington State University, 209 Johnson Hall, 99164-6420, Pullman, WA, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 133-139 0721-7714 25:2<133 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer