caa a22 4500 467901430 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0033-1 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0033-1 An efficient protocol for genetic transformation and shoot regeneration of turmeric ( Curcuma longa L.) via particle bombardment [Elektronische Daten] [Mrudul Shirgurkar, Vaishali Naik, Sara von Arnold, Rajani Nadgauda, David Clapham] Turmeric (Curcuma longa L.) is an important spice crop plant that is sterile and cannot be improved by conventional breeding. An efficient method for stable transformation for turmeric, C. longa L., was developed using particle bombardment. Callus cultures initiated from shoots were bombarded with gold particles coated with plasmid pAHC25 containing the bar and gusA genes each driven by the maize ubiquitin promoter. Transformants were selected on medium containing glufosinate. Transgenic lines were established on selection medium from 50% of the bombarded calluses. Transgenic shoots regenerated from these were multiplied and stably transformed plantlets were produced. Polymerase chain reaction (PCR) and histochemical GUS assay confirmed the stable transformation. Transformed plantlets were resistant to glufosinate. Springer-Verlag, 2005 Curcuma longa L nationallicence Glufosinate nationallicence Particle bombardment nationallicence Stable transformation nationallicence Transgenic plantlets nationallicence Turmeric nationallicence Shirgurkar Mrudul Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut Naik Vaishali Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden aut Nadgauda Rajani Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 112-116 0721-7714 25:2<112 2006 25 299 https://doi.org/10.1007/s00299-005-0033-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0033-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shirgurkar Mrudul Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut NATIONALLICENCE 700 1- Naik Vaishali Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut NATIONALLICENCE 700 1- von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Nadgauda Rajani Tissue Culture Pilot Plant, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, 411008, Pune, India aut NATIONALLICENCE 700 1- Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, Box 7027, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 112-116 0721-7714 25:2<112 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer