caa a22 4500 467901449 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0038-9 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0038-9 Transformation of Montmorency sour cherry ( Prunus cerasus L.) and Gisela 6 ( P. cerasus × P. canescens ) cherry rootstock mediated by Agrobacterium tumefaciens [Elektronische Daten] [Guo-Qing Song, K. Sink] Sour cherry (Prunus cerasus L.) scion cv. Montmorency and rootstock cv. Gisela 6 (P. cerasus × P. canescens) were transformed using Agrobacterium tumefaciens strain EHA105:pBISN1 carrying the neomycin phosphotransferase gene (nptII) and an intron interrupted ß-glucuronidase (GUS) reporter gene (gusA). Whole leaf explants were co-cultivated with A. tumefaciens, and selection and regeneration of transformed cells and shoots of both cultivars was carried out for 12 weeks on selection medium containing 50mgl−1 kanamycin (Km) and 250mgl−1 timentin. These media were [Quoirin and Lepoivre (Acta Hortic 78:437-442, 1977)] supplemented with 0.5mgl−1 benzylaminopurine (BA) + 0.05mgl−1 indole-3-butyric acid (IBA), and woody plant medium [Lloyd and McCown (Proc Int Plant Prop Soc 30:421-427, 1980)] containing 2.0mgl−1 BA + 1.0mgl−1 IBA for cv. Montmorency and cv. Gisela 6, respectively. Seven out of 226 (3.1%) explants of cv. Montmorency and five out of 152 (3.9%) explants of cv. Gisela 6 produced 30/39 GUS- and PCR-positive shoots from the cut midribs via an intermediate callus. Southern analysis of the GUS- and PCR-positive transformants confirmed stable integration of the transgenes with 1-3 copy numbers in the genomes of seven lines of cv. Montmorency and five of cv. Gisela 6. The selected transformants have a normal phenotype in vitro. Springer-Verlag, 2005 Agrobacterium nationallicence Sour cherry ( Prunus cerasus ) nationallicence Sweet cherry ( Prunus avium ) nationallicence Transformation nationallicence Woody plant nationallicence AS : Acetosyringone nationallicence BA : Benzylamino purine nationallicence GUS : β-Glucuronidase nationallicence IBA : Indole-3-butyric acid nationallicence Km : Kanamycin nationallicence MS : Murashige and Skoog medium nationallicence NPTII : Neomycin phosphotransferase nationallicence PCR : Polymerase chain reaction nationallicence TDZ : Thidiazuron nationallicence WPM : Lloyd and McCown woody plant medium nationallicence Song Guo-Qing Department of Horticulture, Michigan State University, Plant Transformation Center, East Lansing, 48824, Michigan, MI, USA aut Sink K. Department of Horticulture, Michigan State University, Plant Transformation Center, East Lansing, 48824, Michigan, MI, USA aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 117-123 0721-7714 25:2<117 2006 25 299 https://doi.org/10.1007/s00299-005-0038-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0038-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Guo-Qing Department of Horticulture, Michigan State University, Plant Transformation Center, East Lansing, 48824, Michigan, MI, USA aut NATIONALLICENCE 700 1- Sink K. Department of Horticulture, Michigan State University, Plant Transformation Center, East Lansing, 48824, Michigan, MI, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 117-123 0721-7714 25:2<117 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer