caa a22 4500 467901465 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20060301xx s 000 0 eng 10.1007/s00299-005-0022-4 doi (NATIONALLICENCE)springer-10.1007/s00299-005-0022-4 Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase [Elektronische Daten] [Chenna Aswath, Sung Mo, Doo Kim, S. Park] A new selection system for onion transformation that does not require the use of antibiotics or herbicides was developed. The selection system used the Escherichia coli gene that encodes phosphomannose isomerase (pmi). Transgenic plants carrying the manA gene that codes for pmi can detoxify mannose-6-phosphate by conversion to fructose-6-phosphate, an intermediate of glycolysis, via the pmi activity. Six-week-old embryogenic callus initiated from seedling radicle was used for transformation. Transgenic plants were produced efficiently with transformation rates of 27 and 23% using Agrobacterium and biolistic system, respectively. Untransformed shoots were eliminated by a stepwise increase from 10gl−1 sucrose with 10gl−1 mannose in the first selection to only10gl−1 mannose in the second selection. Integrative transformation was confirmed by PCR, RT-PCR and Southern hybridization. Springer-Verlag, 2005 Onion nationallicence Transformation nationallicence Phosphomannose- isomerase nationallicence Positive selection nationallicence pmi : Phosphomannose isomerase nationallicence 2,4-D : 2,4-Dichlorophenoxy acetic acid nationallicence ABA : Abscisic acid nationallicence Aswath Chenna Indian Institute of Horticultural Research, 560089, Bangalore, India aut Mo Sung Department of Horticultural Science, Konkuk University, Seoul, South Korea aut Kim Doo Department of Horticultural Science, Konkuk University, Seoul, South Korea aut Park S. Department of Horticultural Science, Konkuk University, Seoul, South Korea aut Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 92-99 0721-7714 25:2<92 2006 25 299 https://doi.org/10.1007/s00299-005-0022-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-005-0022-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aswath Chenna Indian Institute of Horticultural Research, 560089, Bangalore, India aut NATIONALLICENCE 700 1- Mo Sung Department of Horticultural Science, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Doo Department of Horticultural Science, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 700 1- Park S. Department of Horticultural Science, Konkuk University, Seoul, South Korea aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/2(2006-03-01), 92-99 0721-7714 25:2<92 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 467900329 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer