caa a22 4500 467901473 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0183-9 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0183-9 Expression of recombinant Digitalis lanata EHRH. cardenolide 16′-O-glucohydrolase in Cucumis sativus L. hairy roots [Elektronische Daten] [He-Ping Shi, Peter Lindemann] The coding sequence for the Digitalis lanata EHRH. cardenolide 16′-O-glucohydrolase was inserted downstream of the 35S promoter in the binary vector pBI121 resulting in plant expression vector pBI121cgh. Cotyledon explants excised from 10-day-old seedlings of Cucumis sativus L. were transformed using Agrobacterium rhizogenes 15834 harbouring pBI121cgh. Hairy roots were obtained from infected cotyledon explants in vitro 10 days after inoculation. PCR amplification of coding sequences for cgh I, rolB and rolC from Ri plasmid showed that the aimed sequences were inserted into the genome of transformed cucumber hairy roots. Glycolytic activity of the transgenic CGH I was measured by HPLC using Lanatoside glycosides as substrate. Therefore, the cgh I transformed cucumber hairy roots may provide a valuable model for biotransformation of natural compounds by recombinant enzymes. Springer-Verlag, 2006 Digitalis lanata EHRH nationallicence Cucumis sativus L nationallicence CGHI (cardenolide 16′-O-glucohydrolase) nationallicence Hairy roots: Agrobacterium rhizogenes nationallicence Beta-Glucohydrolase nationallicence Shi He-Ping Institut für Pharmakologie und Pharmazeutische Biologie, Martin-Luther—Universität, Hoher Weg 8, 06120, Halle (Saale), Germany aut Lindemann Peter Institut für Pharmakologie und Pharmazeutische Biologie, Martin-Luther—Universität, Hoher Weg 8, 06120, Halle (Saale), Germany aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1193-1198 0721-7714 25:11<1193 2006 25 299 https://doi.org/10.1007/s00299-006-0183-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0183-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi He-Ping Institut für Pharmakologie und Pharmazeutische Biologie, Martin-Luther—Universität, Hoher Weg 8, 06120, Halle (Saale), Germany aut NATIONALLICENCE 700 1- Lindemann Peter Institut für Pharmakologie und Pharmazeutische Biologie, Martin-Luther—Universität, Hoher Weg 8, 06120, Halle (Saale), Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1193-1198 0721-7714 25:11<1193 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer