caa a22 4500 467901481 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0176-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0176-8 Transgenic Acacia sinuata from Agrobacterium tumefaciens -mediated transformation of hypocotyls [Elektronische Daten] [G. Vengadesan, S. Amutha, M. Muruganantham, R. Anand, A. Ganapathi] Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100μM acetosyringone and shoots regenerated on MS (Murashige and Skoog, 1962, Physiol Plant 15:473-497) medium with 13.3μM benzylaminopurine, 2.6μM indole-3-acetic acid, 1gl−1 activated charcoal, 1.5mgl−1 phosphinothricin, and 300mgl−1 cefotaxime. Phosphinothricin at 1.5mgl−1 was used for the selection. Shoots surviving selection on medium with phosphinothricin expressed GUS. Following Southern hybridization, eight independent shoots regenerated of 500 cocultivated explants were demonstrated to be transgenic, which represented transformation frequency of 1.6%. The transgenics carried one to four copies of the transgene. Transgenic shoots were propagated as microcuttings in MS medium with 6.6μM 6-benzylaminopurine and 1.5mgl−1 phosphinothricin. Shoots elongated and rooted in MS medium with gibberellic acid and indole-3-butyric acid, respectively both supplemented with 1.5mgl−1 phosphinothricin. Micropropagation of transgenic plants by microcuttings proved to be a simple means to bulk up the material. Several transgenic plants were found to be resistant to leaf painting with the herbicide Basta. Springer-Verlag, 2006 Agrobacterium transformation nationallicence Acetosyringone nationallicence GUS nationallicence Leguminous tree nationallicence Microcuttings nationallicence Phosphinothricin nationallicence Vengadesan G. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut Amutha S. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut Muruganantham M. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut Anand R. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut Ganapathi A. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1174-1180 0721-7714 25:11<1174 2006 25 299 https://doi.org/10.1007/s00299-006-0176-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0176-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vengadesan G. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut NATIONALLICENCE 700 1- Amutha S. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut NATIONALLICENCE 700 1- Muruganantham M. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut NATIONALLICENCE 700 1- Anand R. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut NATIONALLICENCE 700 1- Ganapathi A. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620024, Tiruchirappalli, Tamilnadu, India aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1174-1180 0721-7714 25:11<1174 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer