caa a22 4500 467901503 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0187-5 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0187-5 Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz [Elektronische Daten] [Hetian Lei, Jianjun Qi, Jingyuan Song, Dejun Yang, Yanzhi Wang, Yinling Zhang, Junshan Yang] Trichosanthin (TCS) from Trichosanthes kirilowii Maximowicz (T. kirilowii) can be used to treat choriocarcinoma. In this work, we established a novel system to produce TCS in crown gall tissues of T. kirilowii infected by Agrobacterium tumefaciens C58 (A. tumefaciens). In the crown gall tissues, a nopaline synthase (NOS) gene of A. tumefaciens was identified by polymerase chain reaction (PCR), and nopaline accumulation was confirmed by a high-voltage filter paper electrophoresis. Furthermore, we optimized conditions to culture the crown gall tissues able to grow fast and produce TCS in an auxin-free medium, and found that a fungal elicitor of Armillaria mellea was capable of stimulation of TCS secretion into the medium. Moreover, we identified that the TCS purified from the crown gall tissues could induce gastric cancer cell death. These data underscore the usefulness of our system as an inexpensive and virtually unlimited source of TCS. Springer-Verlag, 2006 Trichosanthes kirilowii Maximowicz nationallicence Agrobacterium tumefaciens nationallicence Crown gall tissues nationallicence Trichosanthin nationallicence Elicitor nationallicence Inhibition nationallicence Gastric cancer cells nationallicence Lei Hetian Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut Qi Jianjun Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut Song Jingyuan Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut Yang Dejun Department of Chemistry and Life Science, Xiangnan College, 423000, Chenzhou City, Hunan Province, P. R. China aut Wang Yanzhi Lucheng Middle School, Daxing District, 100260, Beijing, P. R. China aut Zhang Yinling Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut Yang Junshan Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1205-1212 0721-7714 25:11<1205 2006 25 299 https://doi.org/10.1007/s00299-006-0187-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0187-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lei Hetian Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut NATIONALLICENCE 700 1- Qi Jianjun Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut NATIONALLICENCE 700 1- Song Jingyuan Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut NATIONALLICENCE 700 1- Yang Dejun Department of Chemistry and Life Science, Xiangnan College, 423000, Chenzhou City, Hunan Province, P. R. China aut NATIONALLICENCE 700 1- Wang Yanzhi Lucheng Middle School, Daxing District, 100260, Beijing, P. R. China aut NATIONALLICENCE 700 1- Zhang Yinling Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut NATIONALLICENCE 700 1- Yang Junshan Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, 100094, Hai-Dian District, Beijing, P. R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1205-1212 0721-7714 25:11<1205 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer