caa a22 4500 467901511 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0189-3 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0189-3 Isolation of two populations of sperm cells from the pollen tube of Torenia fournieri [Elektronische Daten] [Su Chen, Jing Liao, An Kuang, Hui Tian] The two sperm cells of Torenia fournieri are dimorphic. The dimorphic character suggests that they might be preferentially involved in fertilization during in vivo fusion with the egg cell and central cell. To probe the mechanism of preferential fertilization, it is necessary to use the most current molecular techniques. For this purpose, populations of >1000 individuals of the two dimorphic sperm cells, Sua (unassociated with the vegetative nucleus) and Svn (associated with the vegetative nucleus) were isolated from pollen tubes that had grown out of the cut ends of the styles. The two sperm cells released from pollen tubes remained attached to one another. When the two attached sperm cells were transferred into a solution containing 0.01% cellulose, 0.01% pectinase, and 5% mannitol, the connection between the two cells disappeared, and they were easily separated using a micromanipulator. The collection of these two individual populations containing over a thousand cells will permit research on gametic recognition at the molecular level. Springer-Verlag, 2006 Heterospermy nationallicence Dimorphic sperm cells nationallicence Torenia fournieri nationallicence Chen Su South China Botanical Garden, The Chinese Academy of Sciences, 510650, Guangzhou, China aut Liao Jing South China Botanical Garden, The Chinese Academy of Sciences, 510650, Guangzhou, China aut Kuang An Department of Biology, The University of Texas-Pan American, 1201 West University Dr., 78541, Edinburg, TX, USA aut Tian Hui School of Life Sciences, Xiamen University, 361005, Xiamen, China aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1138-1142 0721-7714 25:11<1138 2006 25 299 https://doi.org/10.1007/s00299-006-0189-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0189-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Su South China Botanical Garden, The Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 700 1- Liao Jing South China Botanical Garden, The Chinese Academy of Sciences, 510650, Guangzhou, China aut NATIONALLICENCE 700 1- Kuang An Department of Biology, The University of Texas-Pan American, 1201 West University Dr., 78541, Edinburg, TX, USA aut NATIONALLICENCE 700 1- Tian Hui School of Life Sciences, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1138-1142 0721-7714 25:11<1138 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer