caa a22 4500 46790152X CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0163-0 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0163-0 Genes expressed during the development and ripening of watermelon fruit [Elektronische Daten] [A. Levi, A. Davis, A. Hernandez, P. Wechter, J. Thimmapuram, T. Trebitsh, Y. Tadmor, N. Katzir, V. Portnoy, S. King] A normalized cDNA library was constructed using watermelon flesh mRNA from three distinct developmental time-points and was subtracted by hybridization with leaf cDNA. Random cDNA clones of the watermelon flesh subtraction library were sequenced from the 5′ end in order to identify potentially informative genes associated with fruit setting, development, and ripening. One-thousand and forty-six 5′-end sequences (expressed sequence tags; ESTs) were assembled into 832 non-redundant sequences, designated as "EST-unigenes”. Of these 832 "EST-unigenes”, 254 (∼30%) have no significant homology to sequences published so far for other plant species. Additionally, 168 "EST-unigenes” (∼20%) correspond to genes with unknown function, whereas 410 "EST-unigenes” (∼50%) correspond to genes with known function in other plant species. These "EST-unigenes” are mainly associated with metabolism, membrane transport, cytoskeleton synthesis and structure, cell wall formation and cell division, signal transduction, nucleic acid binding and transcription factors, defense and stress response, and secondary metabolism. This study provides the scientific community with novel genetic information for watermelon as well as an expanded pool of genes associated with fruit development in watermelon. These genes will be useful targets in future genetic and functional genomic studies of watermelon and its development. Springer-Verlag, 2006 cDNA nationallicence Citrullus nationallicence Expressed sequence tags nationallicence Gene expression nationallicence Watermelon nationallicence Levi A. USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, 29414, Charleston, SC, USA aut Davis A. USDA, ARS, P.O. Box 159, 74555, Lane, OK, USA aut Hernandez A. University of Illinois at Urbana-Champaign, Roy J. Carver Biotechnology Center, W.M. Keck Centerfor Comparative and Functional Genomics, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Wechter P. USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, 29414, Charleston, SC, USA aut Thimmapuram J. University of Illinois at Urbana-Champaign, Roy J. Carver Biotechnology Center, W.M. Keck Centerfor Comparative and Functional Genomics, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut Trebitsh T. Department of Life Sciences, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut Tadmor Y. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut Katzir N. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut Portnoy V. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut King S. Department of Horticulture, Texas A&M University, 77845, College Station, TX, USA aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1233-1245 0721-7714 25:11<1233 2006 25 299 https://doi.org/10.1007/s00299-006-0163-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0163-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Levi A. USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, 29414, Charleston, SC, USA aut NATIONALLICENCE 700 1- Davis A. USDA, ARS, P.O. Box 159, 74555, Lane, OK, USA aut NATIONALLICENCE 700 1- Hernandez A. University of Illinois at Urbana-Champaign, Roy J. Carver Biotechnology Center, W.M. Keck Centerfor Comparative and Functional Genomics, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Wechter P. USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, 29414, Charleston, SC, USA aut NATIONALLICENCE 700 1- Thimmapuram J. University of Illinois at Urbana-Champaign, Roy J. Carver Biotechnology Center, W.M. Keck Centerfor Comparative and Functional Genomics, 1201 W. Gregory Drive, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Trebitsh T. Department of Life Sciences, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel aut NATIONALLICENCE 700 1- Tadmor Y. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut NATIONALLICENCE 700 1- Katzir N. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut NATIONALLICENCE 700 1- Portnoy V. Agricultural Research Organization, P.O. Box 1021, 30095, Ramat Yishay, Israel aut NATIONALLICENCE 700 1- King S. Department of Horticulture, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1233-1245 0721-7714 25:11<1233 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer