caa a22 4500 467901554 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0214-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0214-6 Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese [Elektronische Daten] [Chang Kim, Jeung-Sul Han, Hyun-Suk Lee, Joung-Youl Oh, Thoshiro Shigaki, Sung Park, Kendal Hirschi] Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the "B” domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50-65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals. Springer-Verlag, 2006 Arabidopsis nationallicence Calcium nationallicence H+/Ca2+ antiporter nationallicence Manganese nationallicence Potato nationallicence Kim Chang Department of Horticulture, Sangju National University, 742-711, Sangju, Korea aut Han Jeung-Sul National Horticultural Research Institute, Rural Development Administration, 441-440, Suwon, Korea aut Lee Hyun-Suk Department of Horticultural Research, Gyeongbuk Agricultural Technology Administration, 702-708, Daegu, Korea aut Oh Joung-Youl Department of Horticulture, Sangju National University, 742-711, Sangju, Korea aut Shigaki Thoshiro Baylor College of Medicine, Departments of Pediatrics and Human and Molecular Genetics, USDA/ARS Children's Nutrition Research Center, 1100 Bates Street, 77030, Houston, TX, USA aut Park Sung Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut Hirschi Kendal Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1226-1232 0721-7714 25:11<1226 2006 25 299 https://doi.org/10.1007/s00299-006-0214-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0214-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Chang Department of Horticulture, Sangju National University, 742-711, Sangju, Korea aut NATIONALLICENCE 700 1- Han Jeung-Sul National Horticultural Research Institute, Rural Development Administration, 441-440, Suwon, Korea aut NATIONALLICENCE 700 1- Lee Hyun-Suk Department of Horticultural Research, Gyeongbuk Agricultural Technology Administration, 702-708, Daegu, Korea aut NATIONALLICENCE 700 1- Oh Joung-Youl Department of Horticulture, Sangju National University, 742-711, Sangju, Korea aut NATIONALLICENCE 700 1- Shigaki Thoshiro Baylor College of Medicine, Departments of Pediatrics and Human and Molecular Genetics, USDA/ARS Children's Nutrition Research Center, 1100 Bates Street, 77030, Houston, TX, USA aut NATIONALLICENCE 700 1- Park Sung Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 700 1- Hirschi Kendal Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1226-1232 0721-7714 25:11<1226 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer