caa a22 4500 467901562 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0172-z doi (NATIONALLICENCE)springer-10.1007/s00299-006-0172-z Transgenic peas ( Pisum sativum ) expressing polygalacturonase inhibiting protein from raspberry ( Rubus idaeus ) and stilbene synthase from grape ( Vitis vinifera ) [Elektronische Daten] [A. Richter, H.-J. Jacobsen, A. de Kathen, G. de Lorenzo, K. Briviba, R. Hain, G. Ramsay, H. Kiesecker] The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated. Springer-Verlag, 2006 Agrobacterium nationallicence Expression stability nationallicence Pea nationallicence PGIP nationallicence Resveratrol nationallicence Richter A. Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany aut Jacobsen H.-J Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany aut de Kathen A. de Kathen & Pickardt BioTechConsult GbR, Pestalozzi Strasse 35, 10627, Berlin, Germany aut de Lorenzo G. Dip. Biologia Vegetale Università "La Sapienza”, Piazza A. Moro 5, 00185, Roma, Italy aut Briviba K. Institute for Nutritional Physiology, Federal Research Center for Nutrition, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany aut Hain R. Bayer CropScience GmBH, Global Biology Herbicides Biochemistry, 65926, Frankfurt-am-Main, Germany aut Ramsay G. Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut Kiesecker H. German Collection of Microorganisms and Cell Cultures GmbH, Mascheroder Weg 1b, 38124, Braunschweig, Germany aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1166-1173 0721-7714 25:11<1166 2006 25 299 https://doi.org/10.1007/s00299-006-0172-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0172-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Richter A. Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- Jacobsen H.-J Department of Molecular Genetics, University of Hannover, Herrenhäuserstr 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- de Kathen A. de Kathen & Pickardt BioTechConsult GbR, Pestalozzi Strasse 35, 10627, Berlin, Germany aut NATIONALLICENCE 700 1- de Lorenzo G. Dip. Biologia Vegetale Università "La Sapienza”, Piazza A. Moro 5, 00185, Roma, Italy aut NATIONALLICENCE 700 1- Briviba K. Institute for Nutritional Physiology, Federal Research Center for Nutrition, Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Hain R. Bayer CropScience GmBH, Global Biology Herbicides Biochemistry, 65926, Frankfurt-am-Main, Germany aut NATIONALLICENCE 700 1- Ramsay G. Scottish Crop Research Institute, DD2 5DA, Invergowrie, Dundee, UK aut NATIONALLICENCE 700 1- Kiesecker H. German Collection of Microorganisms and Cell Cultures GmbH, Mascheroder Weg 1b, 38124, Braunschweig, Germany aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1166-1173 0721-7714 25:11<1166 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer