caa a22 4500 467901570 CHVBK 20180406152819.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0178-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0178-6 Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter [Elektronische Daten] [Keming Luo, Wei Deng, Yuehua Xiao, Xuelian Zheng, Yi Li, Yan Pei] To extend the shelf life of freshly harvested vegetables and cut flowers, a maize homeobox gene Knotted1 (kn1) was placed under the control of a wound-inducible promoter win3.12 from hybrid poplar (Populus trichocarpa × P. deltoides) and introduced into tobacco plants (Nicotiana tabacum cv. Xanthi). Transgenic win3.12::kn1 plants were morphologically normal. A leaf-detachment assay demonstrated that senescence in win3.12::kn1 leaves could be delayed by at least 2 weeks compared with wild type leaves. Furthermore, all leaves of win3.12::kn1 shoots remained green and healthy 3 weeks after excision and incubation in water, while older leaves of control shoots senesced under the same conditions. Additionally, a number of adventitious roots produced at the cut ends of wild type shoots after a 3-week incubation, but much a less number of adventitious roots appeared in win3.12::kn1 shoots. The delay in senescence was also confirmed by a higher total chlorophyll (a + b) content in win3.12::kn1 leaves relative to that of the control plants. RT-PCR analysis showed that the kn1 transcript was detected in win3.12::kn1 leaves with wounding treatment, but otherwise was not observed in leaves of wild type and unwounded transgenic plants. The results presented here indicate that expression of kn1 gene driven by the wound-inducible promoter win3.12 is potentially useful to delay senescence of vegetable crops and commercial horticulture after harvest. Springer-Verlag, 2006 Cytokinin nationallicence kn1 nationallicence Senescence nationallicence Wound-inducible promoter nationallicence Luo Keming Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Deng Wei Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Xiao Yuehua Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Zheng Xuelian Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Li Yi Department of Plant Science, University of Connecticut, 1376 Storrs Road U-4067, 06269-4067, Storrs, CT, USA aut Pei Yan Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1246-1254 0721-7714 25:11<1246 2006 25 299 https://doi.org/10.1007/s00299-006-0178-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0178-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Keming Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Deng Wei Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Xiao Yuehua Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Zheng Xuelian Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 700 1- Li Yi Department of Plant Science, University of Connecticut, 1376 Storrs Road U-4067, 06269-4067, Storrs, CT, USA aut NATIONALLICENCE 700 1- Pei Yan Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, 400716, Chongqing, P.R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1246-1254 0721-7714 25:11<1246 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer