caa a22 4500 467901589 CHVBK 20180406152820.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0177-7 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0177-7 Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco [Elektronische Daten] [Riliang Gu, Li Zhao, Ying Zhang, Xiaoping Chen, Juan Bao, Jinfeng Zhao, Zhangying Wang, Junjie Fu, Tingsong Liu, Jianhua Wang, Guoying Wang] The β-glucosidase gene of maize (ZmGLU1) was suggested to hydrolyze cytokinin-conjugate and release free cytokinin during plant growth and development. A clone containing the upstream region of ZmGLU1 was isolated and sequenced from a maize genomic library. The full-length ZmGLU1 promoter and a series of its 5′ deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. The GUS activity of transgenic plants was assayed at various developmental stages. The results showed that ZmGLU1 promoter-driven GUS gene had the highest expression level in the roots and that the expression of GUS gene declined during seed maturation and down to the lowest level in mature seeds. The ZmGLU1 promoter-driven GUS expression increased during seed germination, reaching a peak on day 11. The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA3, ABA, SA, cold, salt, drought, and submergence treatments. The histochemical staining revealed that GUS activity was located in vigorous cell division zones with dominant staining associated with vascular tissues. Deletion analysis showed that the promoter contained a putative leaf-specific and stem-specific negative regulative element and two putative enhancers. Springer-Verlag, 2006 Beta-glucosidase nationallicence Cytokinin nationallicence Expression pattern nationallicence GUS nationallicence Promoter nationallicence Gu Riliang State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Zhao Li State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Zhang Ying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Chen Xiaoping State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Bao Juan State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Zhao Jinfeng State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Zhangying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Fu Junjie State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Liu Tingsong State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Jianhua State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Guoying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1157-1165 0721-7714 25:11<1157 2006 25 299 https://doi.org/10.1007/s00299-006-0177-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0177-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gu Riliang State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhao Li State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhang Ying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Chen Xiaoping State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Bao Juan State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhao Jinfeng State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Zhangying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Fu Junjie State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Liu Tingsong State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Jianhua State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Guoying State Key Laboratory for Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1157-1165 0721-7714 25:11<1157 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer