caa a22 4500 467901619 CHVBK 20180406152820.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0184-8 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0184-8 Transgenic wheat progeny resistant to powdery mildew generated by Agrobacterium inoculum to the basal portion of wheat seedling [Elektronische Daten] [Tong-Jin Zhao, Shuang-Yi Zhao, Hui-Min Chen, Qing-Zhen Zhao, Zan-Min Hu, Bing-Kai Hou, Guang-Min Xia] To improve the transformation efficiency of wheat (Triticum aestivum L.) mediated by Agrobacterium tumefaciens, we explored the possibility of employing the basal portion of wheat seedling (shoot apical meristem) as the explants. Three genotypes of wheat were transformed by A. tumefaciens carrying β-1, 3-glucanase gene. After vernalization, the seeds to be transformed were germinated. When these seedlings grew up to 2∼5cm, their coleoptile and half of the cotyledon were cut out, and the basal portions were infected by A. tumefaciens. A total 27 T0 transgenic plants were obtained, and the average transformation efficiency was as high as 9.82%. Evident segregation occurred in some of the T1 plants, as was indicated by PCR and Southern blotting analysis. Investigation of the T2 plants revealed that some transformed plants had higher resistance to powdery mildew than the controls. Northern blotting revealed that β-1, 3-glucanase gene was normally expressed in the T2 plants, which showed an increased resistance to powdery mildew. The results above indicate that the exogenous gene has been successfully integrated into the genome of wheat, transmitted and expressed in the transgenic progeny. From all the results above, it can be concluded that Agrobacterium inoculum to the basal portion of wheat seedling is a highly efficient and dependable transformation method. It can be developed into a practicable method for transfer of target gene into wheat. Springer-Verlag, 2006 Agrobacterium tumefaciens nationallicence β-1, 3-glucanase gene nationallicence Powdery mildew nationallicence Transformation of shoot apical meristem nationallicence Transgene nationallicence Triticum aestivum nationallicence Zhao Tong-Jin School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Zhao Shuang-Yi School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Chen Hui-Min School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Zhao Qing-Zhen School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Hu Zan-Min Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, P. R. China aut Hou Bing-Kai School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Xia Guang-Min School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1199-1204 0721-7714 25:11<1199 2006 25 299 https://doi.org/10.1007/s00299-006-0184-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0184-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Tong-Jin School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 700 1- Zhao Shuang-Yi School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 700 1- Chen Hui-Min School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 700 1- Zhao Qing-Zhen School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 700 1- Hu Zan-Min Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, P. R. China aut NATIONALLICENCE 700 1- Hou Bing-Kai School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 700 1- Xia Guang-Min School of Life Sciences, Shandong University, 250100, Jinan, P. R. China aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1199-1204 0721-7714 25:11<1199 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer