caa a22 4500 467901635 CHVBK 20180406152820.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s00299-006-0186-6 doi (NATIONALLICENCE)springer-10.1007/s00299-006-0186-6 Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus [Elektronische Daten] [Makoto Tougou, Noriyuki Furutani, Noriko Yamagishi, Yoshiaki Shizukawa, Yoshihito Takahata, Soh Hidaka] In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by β-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T0 plants with an introduced CP gene were obtained, and one generated T1 seeds. The presence of the transgene in T1 plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T2 plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T2 plants achieved resistance to SbDV by an RNA-silencing-mediated process. Springer-Verlag, 2006 Glycine max nationallicence RNA silencing nationallicence SbDV nationallicence Transformation nationallicence Resistance nationallicence Tougou Makoto National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut Furutani Noriyuki Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, 619-0244, Soraku-Gun, Kyoto, Japan aut Yamagishi Noriko National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut Shizukawa Yoshiaki Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, 619-0244, Soraku-Gun, Kyoto, Japan aut Takahata Yoshihito Laboratory of Plant Breeding, Iwate University, 020-8550, Morioka, Iwate, Japan aut Hidaka Soh National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1213-1218 0721-7714 25:11<1213 2006 25 299 https://doi.org/10.1007/s00299-006-0186-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00299-006-0186-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tougou Makoto National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Furutani Noriyuki Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, 619-0244, Soraku-Gun, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamagishi Noriko National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Shizukawa Yoshiaki Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, 619-0244, Soraku-Gun, Kyoto, Japan aut NATIONALLICENCE 700 1- Takahata Yoshihito Laboratory of Plant Breeding, Iwate University, 020-8550, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Hidaka Soh National Agricultural Research Center for Tohoku Region, 020-0198, Morioka, Iwate, Japan aut NATIONALLICENCE 773 0- Plant Cell Reports Springer-Verlag 25/11(2006-11-01), 1213-1218 0721-7714 25:11<1213 2006 25 299 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer