caa a22 4500 467902194 CHVBK 20180406152821.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10661-006-7234-0 doi (NATIONALLICENCE)springer-10.1007/s10661-006-7234-0 The use of bioaccumulation, biomarkers and histopathology diseases in shape Procambarus clarkii to establish bioavailability of Cd and Zn after a mining spill [Elektronische Daten] [M. Martín-Díaz., S. Tuberty, C. McKenney Jr., J. Blasco, C. Sarasquete, T. DelValls] Individuals of the crayfish Procambarus clarkii (males and females) were exposed simultaneously to cadmium and zinc during 21 days. Exposure concentrations were those determined at the Guadiamar river after the Aznalcóllar mining spill (SW, Spain): 10 and 30 μg L−1 of cadmium and 1000 and 3000 μg L−1 of zinc. Three biomarkers (MT: metallothioneins like proteins, VTG: vitellogenin/vitellin like proteins and histopathology) together with heavy metal bioaccumulation were determined in soft tissues of male and female P. clarkii. At the concentrations tested, increasing cadmium exposure resulted in increasing cadmium bioaccumulation and increasing subletal effects (induction of MT, VTG and histopathological damage in tissues). Nevertheless, although increasing zinc exposure showed increasing VTG induction and histopathological damages, not a positive relationship was determined with MT induction. Concerning to responses determined in male and female crayfishes only differences were found between sexes at the highest cadmium exposure concentration related to bioaccumulation in hepatopancreas tissues. Biomarkers responses to heavy metal contamination in this crayfish, even VTG induction not before tested in heavy metal contamination assessment in crustaceans resulted potential tools for the monitoring of heavy metal environmental contamination. Springer Science + Business Media, Inc., 2006 metallothionein nationallicence vitellogenin nationallicence reproduction nationallicence heavy metals nationallicence Martín-Díaz. M. Facultad de Ciencias del Mar y Ambientales, Campus Río San Pedro s/n, 11510, Puerto Real, Spain aut Tuberty S. University of West Florida, Center for Environmental Diagnostics and Bioremediation, 32514, Pensacola, FL, USA aut McKenney Jr. C. US Environmental Protection Agency NHEERL, Gulf Ecology Division, One Sabine Island Dr., 32561, Gulf Breeze, FL, USA aut Blasco J. Instituto de Ciencias Marinas de Andalucía, Campus Río Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut Sarasquete C. Instituto de Ciencias Marinas de Andalucía, Campus Río Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut DelValls T. Facultad de Ciencias del Mar y Ambientales, Campus Río San Pedro s/n, 11510, Puerto Real, Spain aut Environmental Monitoring and Assessment Kluwer Academic Publishers 116/1-3(2006-05-01), 169-184 0167-6369 116:1-3<169 2006 116 10661 https://doi.org/10.1007/s10661-006-7234-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-006-7234-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martín-Díaz M. Facultad de Ciencias del Mar y Ambientales, Campus Río San Pedro s/n, 11510, Puerto Real, Spain aut NATIONALLICENCE 700 1- Tuberty S. University of West Florida, Center for Environmental Diagnostics and Bioremediation, 32514, Pensacola, FL, USA aut NATIONALLICENCE 700 1- McKenney Jr C. US Environmental Protection Agency NHEERL, Gulf Ecology Division, One Sabine Island Dr., 32561, Gulf Breeze, FL, USA aut NATIONALLICENCE 700 1- Blasco J. Instituto de Ciencias Marinas de Andalucía, Campus Río Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Sarasquete C. Instituto de Ciencias Marinas de Andalucía, Campus Río Pedro s/n, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- DelValls T. Facultad de Ciencias del Mar y Ambientales, Campus Río San Pedro s/n, 11510, Puerto Real, Spain aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 116/1-3(2006-05-01), 169-184 0167-6369 116:1-3<169 2006 116 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer