caa a22 4500 467902488 CHVBK 20180406152822.0 cr unu---uuuuu 170328e20060701xx s 000 0 eng 10.1007/s10661-006-0771-8 doi (NATIONALLICENCE)springer-10.1007/s10661-006-0771-8 Faecal Coliform Bacteria in Febros River (Northwest Portugal): Temporal Variation, Correlation with Water Parameters, and Species Identification [Elektronische Daten] [João Cabral, Cristina Marques] Febros river water was sampled weekly, during 35 successive weeks, and analyzed for microbiological (total coliforms, faecal coliforms, faecal streptococci and enterococci) and chemical-physical (ammonia and temperature) parameters. All microbiological parameters were highly correlated with each other and with ammonia, suggesting that the simultaneous determination of all variables currently in use in the evaluation of the microbiological quality of waters is probably redundant, and could be simplified, and that ammonia should be tested as a sentinel parameter of the microbiological pollution load of Febros river. From the strains isolated from positive tubes of the faecal coliforms test (multiple tube fermentation technique) and retested in this assay, Escherichia coli, Klebsiella oxytoca and Klebsiella pneumoniae subsp. pneumoniae strains were positive, indicating that the faecal coliforms test is not totally specific for Escherichia coli, and can detect other bacteria. Considering that these Klebsiella spp. are not necessarily of faecal origin, it was concluded that the faecal coliforms test can overestimate true faecal pollution. From the strains isolated from positive tubes of the faecal coliforms procedure, only Escherichia coli strains were clearly positive in the β-D-glucuronidase test. All other species were negative or very weakly positive, suggesting that the assay of the β-D-glucuronidase activity is less prone to false positives than the faecal coliforms test in the quantification of Escherichia coli in environmental waters. Springer Science + Business Media, Inc., 2006 β-D-glucuronidase nationallicence ammonia nationallicence Escherichia coli nationallicence faecal coliforms nationallicence Klebsiella nationallicence Cabral João Faculty of Sciences, Department of Botany Oporto, University of Oporto, Portugal aut Marques Cristina Faculty of Sciences, Department of Botany Oporto, University of Oporto, Portugal aut Environmental Monitoring and Assessment Kluwer Academic Publishers 118/1-3(2006-07-01), 21-36 0167-6369 118:1-3<21 2006 118 10661 https://doi.org/10.1007/s10661-006-0771-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-006-0771-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cabral João Faculty of Sciences, Department of Botany Oporto, University of Oporto, Portugal aut NATIONALLICENCE 700 1- Marques Cristina Faculty of Sciences, Department of Botany Oporto, University of Oporto, Portugal aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 118/1-3(2006-07-01), 21-36 0167-6369 118:1-3<21 2006 118 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer