caa a22 4500 467902887 CHVBK 20180406152824.0 cr unu---uuuuu 170328e20060601xx s 000 0 eng 10.1007/s10661-006-7670-x doi (NATIONALLICENCE)springer-10.1007/s10661-006-7670-x Toxicity Testing of Leachate from Waste Landfills Using Medaka ( Oryzias Latipes ) for Monitoring Environmental Safety [Elektronische Daten] [Kae Osaki, Shosaku Kashiwada, Norihisa Tatarazako, Yoshiro Ono] To investigate the environmental safety of waste disposal landfill sites and of land reclaimed from such sites, we evaluated the toxicity of leachate from these sites by a combination of bioassays in the Japanese killifish medaka Oryzias latipes. We tested for lethal toxicity in adult and larval medaka and for hatching inhibition of embryos from eggs. As biochemical evidence of the effects of leachate exposure, CYP1A (EROD activity) and vitellogenin (Vtg) were induced. We also bioassayed water-treated leachate and downstream river water. Leachate solution was lethal to larval and adult medaka. Embryo hatchability was inhibited, and abnormal hatching, spinal deformity and anisophthalmia occurred in embryos exposed to leachate solution. CYP1A was induced by exposure to leachate solution diluted to 1.0%, and EROD activity was significantly higher than in control. Vtg and unknown proteins were induced in the sera of male medaka exposed to the diluted leachate solution. Conventional water treatments worked effectively to remove toxic compounds but did not work well to remove element ions, including heavy metals. Treated leachate produced neither lethal toxicity nor hatching abnormalities during the exposure period. Fish toxicity tests for leachate would be useful for monitoring the environmental safety of landfill sites. Springer Science + Business Media, Inc., 2006 ecotoxicity nationallicence hazardous waste nationallicence leachate nationallicence lethal toxicity nationallicence medaka nationallicence waste disposal landfill site nationallicence Osaki Kae Department of Environmental and Civil Engineering, Okayama University, 3-1-1, Tsushima-naka, 700-8530, Okayama, Japan aut Kashiwada Shosaku Nicholas School of the Environment and Earth Sciences, Duke University, 27708-0328, Durham, NC, USA aut Tatarazako Norihisa National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Ibaraki, Japan aut Ono Yoshiro Department of Environmental and Civil Engineering, Okayama University, 3-1-1, Tsushima-naka, 700-8530, Okayama, Japan aut Environmental Monitoring and Assessment Kluwer Academic Publishers 117/1-3(2006-06-01), 73-84 0167-6369 117:1-3<73 2006 117 10661 https://doi.org/10.1007/s10661-006-7670-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-006-7670-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osaki Kae Department of Environmental and Civil Engineering, Okayama University, 3-1-1, Tsushima-naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Kashiwada Shosaku Nicholas School of the Environment and Earth Sciences, Duke University, 27708-0328, Durham, NC, USA aut NATIONALLICENCE 700 1- Tatarazako Norihisa National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Ibaraki, Japan aut NATIONALLICENCE 700 1- Ono Yoshiro Department of Environmental and Civil Engineering, Okayama University, 3-1-1, Tsushima-naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 117/1-3(2006-06-01), 73-84 0167-6369 117:1-3<73 2006 117 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer