caa a22 4500 467903204 CHVBK 20180406152825.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10661-005-9167-4 doi (NATIONALLICENCE)springer-10.1007/s10661-005-9167-4 Standardizing the Methodology of Sperm Cell Test with Paracentrotus Lividus [Elektronische Daten] [S. Lera, S. Macchia, D. Pellegrini] The sperm cell toxicity test with Paracentrotus lividus is widely used in Italy to assess the quality of complex aqueous matrices, but at present there is a shortage of standardized methodologies for the bioassay performance. In this work several critical points were considered, in order to verify the reliability of this bioassay and to improve its standardization. In particular, we have studied the differences in EC50 values at different sperm: egg ratios and with different quantities of gametes; the influence of sperm and eggs on fertilization rate and the influence of different recipients materials (plastic or glass). At the same time, it was evaluated if the obtained EC50 values were in accordance with those reported in literature. Experiments performed yielded EC50 values for copper ranging from 16.54 μ g/l to 69.59 μ g/l, with a total mean value ± SD of 39.79 μ g/l ± 11.17. These data fit with those found in literature for other echinoid species. According to the same authors, poor fertilization was probably due to the effects on sperm and not on eggs, and moreover the linear regression analysis performed on each experiment suggested that the best amount of eggs to use in the test was 1000. Results from the comparison between the performance of the test in plastic or glass tubes showed an higher success in fertilization rate using borosilicate glass tubes. Springer Science+Business Media, Inc., 2006 copper nationallicence gametes nationallicence Paracentrotus lividus nationallicence plastic or glass tubes nationallicence sea urchins nationallicence sperm cell test nationallicence sperm: egg ratio nationallicence standardizing nationallicence Lera S. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut Macchia S. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut Pellegrini D. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut Environmental Monitoring and Assessment Kluwer Academic Publishers 122/1-3(2006-11-01), 101-109 0167-6369 122:1-3<101 2006 122 10661 https://doi.org/10.1007/s10661-005-9167-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-005-9167-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lera S. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut NATIONALLICENCE 700 1- Macchia S. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut NATIONALLICENCE 700 1- Pellegrini D. ICRAM - Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 122/1-3(2006-11-01), 101-109 0167-6369 122:1-3<101 2006 122 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer