caa a22 4500 46790345X CHVBK 20180406152826.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10661-005-9000-0 doi (NATIONALLICENCE)springer-10.1007/s10661-005-9000-0 Evaluation of the Fertilization Capability of Paracentrotus Lividus Sea Urchin Storaged Gametes by the Exposure to Different Aqueous Matrices [Elektronische Daten] [S. Lera, D. Pellegrini] Several experiments were performed to evaluate the fertilization capability of Paracentrotus lividus sea urchin gametes, which are usually used in fertilization tests, but which, until now, were employed only for few hours after spawning. The aim of this work is to evaluate the possibility of performing the bioassay for longer periods, employing ‘conserved' gametes. As far as the method, the sperm was stored ‘dry' at 4 ∘C; eggs were conserved in an antibiotic solution at 4 ∘C, and they were exposed both to copper solutions as reference toxicant and to aqueous matrices. The use of conserved gametes in the fertilization bioassay lead to EC50 values which were acceptable for about 3 days. Moreover, these experiments permitted to identify the limiting factor: the male gamete. Springer Science + Business Media, Inc., 2006 antibiotics nationallicence bioassay nationallicence copper nationallicence gametes storage nationallicence Paracentrotus lividus nationallicence sea urchin nationallicence aqueous matrices nationallicence Lera S. ICRAM, Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut Pellegrini D. ICRAM, Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut Environmental Monitoring and Assessment Kluwer Academic Publishers 119/1-3(2006-08-01), 1-13 0167-6369 119:1-3<1 2006 119 10661 https://doi.org/10.1007/s10661-005-9000-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-005-9000-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lera S. ICRAM, Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut NATIONALLICENCE 700 1- Pellegrini D. ICRAM, Central Institute of Marine Research, Via di Casalotti 300, Rome, Italy aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 119/1-3(2006-08-01), 1-13 0167-6369 119:1-3<1 2006 119 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer