caa a22 4500 467903646 CHVBK 20180406152826.0 cr unu---uuuuu 170328e20060801xx s 000 0 eng 10.1007/s10661-005-9038-z doi (NATIONALLICENCE)springer-10.1007/s10661-005-9038-z Amperometric Detection of Pesticides Using Polymer Electrodes [Elektronische Daten] [Dipankar Ghosh, Kakoli Dutta, Dipankar Bhattacharyay, Priyabrata Sarkar] Abstact: The real time monitoring of some organophosphorus based pesticides is of great concern to environmentalists because the widespread use of pesticides is causing severe health hazards to all living beings and also hampering our ecological balance. The traditional methods of measurement of pesticide residues are time consuming, need sample pre-treatment, and lack desired specificity and accuracy. We have developed an amperometric biosensor for indirect measurement of the pesticide concentration precisely in ppb level. The method is based on the action of two enzymes namely acetylcholine esterase and choline oxidase which are uniquely immobilized in a polymeric porous network directly on the working electrode of a screen-printed sensor. Polyacrylamide matrix has been prepared by copolymerisation of acrylamide andN,N′-methylenebisacrylamide using Potassium peroxodisulphate (K2S2O8) as initiator. A linear relationship was obtained between the range of 0 to 10 ppb. Springer Science+Business Media, Inc., 2006 amperometric nationallicence biosensor nationallicence pesticide nationallicence polyacrylamide nationallicence screen-printed electrode nationallicence Ghosh Dipankar Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut Dutta Kakoli Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut Bhattacharyay Dipankar Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut Sarkar Priyabrata Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut Environmental Monitoring and Assessment Kluwer Academic Publishers 119/1-3(2006-08-01), 481-489 0167-6369 119:1-3<481 2006 119 10661 https://doi.org/10.1007/s10661-005-9038-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-005-9038-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghosh Dipankar Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut NATIONALLICENCE 700 1- Dutta Kakoli Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut NATIONALLICENCE 700 1- Bhattacharyay Dipankar Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut NATIONALLICENCE 700 1- Sarkar Priyabrata Department of Polymer Science and Technology, University of Calcutta, 92 APC Road, 700009, Kolkata, India aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Kluwer Academic Publishers 119/1-3(2006-08-01), 481-489 0167-6369 119:1-3<481 2006 119 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer