caa a22 4500 467904057 CHVBK 20180406152828.0 cr unu---uuuuu 170328e20061001xx s 000 0 eng 10.1007/s10661-005-9121-5 doi (NATIONALLICENCE)springer-10.1007/s10661-005-9121-5 A Comparison of Bacterial Indicators and Methods in Rural Surface Waters [Elektronische Daten] [Robin Kloot, Bobby Radakovich, Xiaoqing Huang, Daniel Brantley] The United States Environmental Protection Agency (USEPA) recommends the use of Escherichia coli (E. coli) and enterococci as indicators of enteric pathogens in fresh waters; however, fecal coliform analyses will remain important by virtue of the large amount of historic data collected in prior years. In this study, we attempted, in a real-world situation (i.e., a rural inland watershed in the Piedmont of South Carolina) to compare different bacterial indicators and methods to one another. We compared fecal coliforms, enumerated by membrane filtration with E. coli, enumerated by a commercialized enzyme substrate method and observed E. coli/fecal coliform ratios of 1.63 and 1.2 for two separate tests. In the same watershed, we observed an E. coli/fecal coliform ratio of 0.84 when we used the commercialized enzyme substrate method for both enumerations. Given these results, users of such data should exercise care when they make comparisons between historic membrane filtration data and data acquired through the use of the more modern enzymatic methods. Some sampling and side-by-side testing between methods in a specific watershed may be prudent before any conversion factors between old and new datasets are applied. Springer Science+Business Media, Inc., 2006 Escherichia coli nationallicence fecal coliform nationallicence bacteria nationallicence indicator nationallicence methods nationallicence rural nationallicence watershed nationallicence Kloot Robin Earth Sciences and Resources Institute at the University of South Carolina, 402 Byrnes Building, 29208, Columbia, SC, USA aut Radakovich Bobby LT Environmental, 4400 W. 46th Ave, 80212, Denver, CO, USA aut Huang Xiaoqing Department of Biology, West Virginia University, 26506, Morgantown, WV, USA aut Brantley Daniel Earth Sciences and Resources Institute at the University of South Carolina, 402 Byrnes Building, 29208, Columbia, SC, USA aut Environmental Monitoring and Assessment Springer Netherlands 121/1-3(2006-10-01), 275-287 0167-6369 121:1-3<275 2006 121 10661 https://doi.org/10.1007/s10661-005-9121-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10661-005-9121-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kloot Robin Earth Sciences and Resources Institute at the University of South Carolina, 402 Byrnes Building, 29208, Columbia, SC, USA aut NATIONALLICENCE 700 1- Radakovich Bobby LT Environmental, 4400 W. 46th Ave, 80212, Denver, CO, USA aut NATIONALLICENCE 700 1- Huang Xiaoqing Department of Biology, West Virginia University, 26506, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Brantley Daniel Earth Sciences and Resources Institute at the University of South Carolina, 402 Byrnes Building, 29208, Columbia, SC, USA aut NATIONALLICENCE 773 0- Environmental Monitoring and Assessment Springer Netherlands 121/1-3(2006-10-01), 275-287 0167-6369 121:1-3<275 2006 121 10661 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer