caa a22 4500 467905460 CHVBK 20180406152832.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10735-006-9046-5 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9046-5 WWOX protein expression in normal human tissues [Elektronische Daten] [Maria Nunez, John Ludes-Meyers, C. Aldaz] WWOX is a putative tumor suppressor gene that spans approximately a 1Mb genomic region and is the site for the second most common chromosomal fragile site, FRA16D at 16q23. Various studies have focused on the expression of WWOX in human cancer mostly at the RNA level, but little is known about the normal pattern of WWOX protein expression in non-neoplastic tissues. In this study, a comprehensive analysis of WWOX protein expression in normal tissues was performed by means of immunohistochemistry utilizing a very specific anti-WWOX polyclonal antibody. We analyzed tissue cores of human samples representing more than 30 organs, using various tissue microarray (TMA) slides. Due to the potential role of WWOX in sex-steroid metabolism, whole sections from hormonally regulated organs like breast, ovaries, testes and prostate were also analyzed. The results from our study indicate that WWOX is preferentially highly expressed in secretory epithelial cells of reproductive, endocrine and exocrine organs, as well as in ductal epithelial cells from specific segments of the urinary system. Interestingly, we also observed significant WWOX protein expression in various cell types of neural origin including neurons, ependymal cells and astrocytes. No expression of WWOX was detected in adipose, connective, and lymphoid tissues, myelinized structures and blood vessels. By better defining the topographic distribution of WWOX in normal tissues this study provides some insight on the potential physiological role of this novel protein. Springer Science+Business Media B.V., 2006 WWOX nationallicence Protein expression nationallicence Tissue Microarrays nationallicence Tumor suppressor nationallicence FRA16D nationallicence Nunez Maria Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut Ludes-Meyers John Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut Aldaz C. Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 115-125 1567-2379 37:3-4<115 2006 37 10735 https://doi.org/10.1007/s10735-006-9046-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9046-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nunez Maria Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut NATIONALLICENCE 700 1- Ludes-Meyers John Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut NATIONALLICENCE 700 1- Aldaz C. Department of Carcinogenesis, M.D. Anderson Cancer Center, Science Park-Research Division, The University of Texas, P.O. Box 389, 78957, Smithville, TX, USA aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 115-125 1567-2379 37:3-4<115 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer