caa a22 4500 467905487 CHVBK 20180406152832.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10735-006-9053-6 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9053-6 Quantitative immunogold study of increased expression of metallothionein-I/II in the brain perivascular areas of diabetic scrapie-infected mice [Elektronische Daten] [Andrzej Vorbrodt, Danuta Dobrogowska, Harry Meeker, Richard Carp] Quantitative immunogold procedure was used to study the distribution of metallothionein I/II (MT-I/II) at the ultrastructural level in the perivascular areas, including microvascular endothelial cells (ECs) and astrocytes with their perivascular end-feet, in brains of scrapie-infected hyperglycemic (diabetic) and normoglycemic (non-diabetic) mice. Samples of the fronto-parietal cortex obtained from diabetic and non-diabetic scrapie-infected, as well as from non-infected (control) SJL/J mice, were processed for immunocytochemical examination. In control mice, the labelling of the ECs was of low intensity, restricted to few immunogold particles in the cytoplasm. More intense labelling was present in the cytoplasm of astrocytic perivascular processes and perikarya, where it was associated with endoplasmic reticulum and fibrils. A few immunosignals were also present inside the nuclei of astrocytes. In diabetic mice the labelling of the EC cytoplasm was slightly increased, whereas in the cytoplasm of perivascular processes and pericarya of astrocytes, including their nuclei, there was significant enhancement of labelling. In these cells the density of immunosignals was highest in the areas of cytoplasm containing bundles of fibrils. In non-diabetic, scrapie-infected mice the intensity of immunolabelling was higher than in control mice but slightly lower than in diabetic mice. These results are similar to those in Alzheimer's disease reported by other authors, and suggest that neurodegenerative diseases as well as metabolic stress enhance the metallothionein expression in perivascular regions of brain cerebral cortex, predominantly in astrocytes. Springer Science+Business Media, Inc., 2006 Blood-brain barrier nationallicence Immunogold cytochemistry nationallicence Endothelial cells nationallicence Perivascular end-feet nationallicence Pathologic stress nationallicence Metabolic stress nationallicence Vorbrodt Andrzej Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut Dobrogowska Danuta Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut Meeker Harry Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut Carp Richard Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 143-151 1567-2379 37:3-4<143 2006 37 10735 https://doi.org/10.1007/s10735-006-9053-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9053-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vorbrodt Andrzej Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut NATIONALLICENCE 700 1- Dobrogowska Danuta Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut NATIONALLICENCE 700 1- Meeker Harry Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut NATIONALLICENCE 700 1- Carp Richard Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, New York, NY, USA aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 143-151 1567-2379 37:3-4<143 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer