caa a22 4500 467905517 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10735-006-9058-1 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9058-1 Morphological features of osteoclasts derived from a co-culture system [Elektronische Daten] [Vanessa Nicolin, Giovanna Baldini, Renato Bareggi, Marina Zweyer, Giorgio Zauli, Mauro Vaccarezza, Paola Narducci] The interaction between the receptor activator of NfKB (RANK) and its ligand receptor activator of NfKB ligand (RANKL) has recently been proven to be pivotal for osteoclast differentiation and activation. The influence of RANK-RANKL signaling on osteoclast formation was established by co-culturing murine osteoblasts (type CRL-12257) and murine mononuclear monocytes (RAW 264.7). The aim of the present study was to examine, by means of morphological techniques, the interaction between these two cell lines grown in the absolute absence of exogenous cytokines and other stimulating factors. Moreover, we wanted to show that our model could provide a system to analyze the bone resorption process. Mineralized matrix induced morphological changes of osteoclasts (OC) by the formation of organized ruffled-border and a large number of secondary lysosomal vesicles. On the contrary, OC grown on glass coverslips without dentin showed no organized ruffled border or secondary lysosomes. The study of the relationship between these two cell types could establish new approaches for a potential pharmacological control of these cell types and tissues in health and disease. Springer Science+Business Media, Inc., 2006 Osteoclast nationallicence Pre-osteoclast nationallicence Receptor activator of NfKB ligand nationallicence Transmission electron microscopy nationallicence Scanning electron microscopy nationallicence Nicolin Vanessa Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Baldini Giovanna Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Bareggi Renato Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Zweyer Marina Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Zauli Giorgio Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Vaccarezza Mauro Department of Health and Human Movement Sciences, University of Cassino, Viale Bonomi, 03043, Cassino (FR), Italy aut Narducci Paola Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 171-177 1567-2379 37:3-4<171 2006 37 10735 https://doi.org/10.1007/s10735-006-9058-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9058-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nicolin Vanessa Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 700 1- Baldini Giovanna Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 700 1- Bareggi Renato Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 700 1- Zweyer Marina Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 700 1- Zauli Giorgio Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 700 1- Vaccarezza Mauro Department of Health and Human Movement Sciences, University of Cassino, Viale Bonomi, 03043, Cassino (FR), Italy aut NATIONALLICENCE 700 1- Narducci Paola Department of Biomedicine, Section of Human Morphology and Molecular Biology, University of Trieste, Via manzoni,16, 34138, Trieste, Italy aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 171-177 1567-2379 37:3-4<171 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer