caa a22 4500 467905525 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10735-006-9057-2 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9057-2 Thymus development in Macaca fascicularis (Cynomolgus monkey): an approach for toxicology and embryology [Elektronische Daten] [Eberhard Buse, Gunnar Habermann, Friedhelm Vogel] Thymus development was studied in the cynomolgus monkey from day35 of gestation (gd35) to the stage of advanced involution in a 21-year-old monkey. Special emphasis was placed on thymus cell generation and cellular pattern formation. At gd35, the epithelial bud of the thymus was visible in a sagittal position at the level of the thoracic aperture. At gd50, first lymphocyte-like cells and few Human Leukocyte Antigen-D Region (HLA-DR) immunoreactive cells appeared. The cortico-medullary differentiation, Hassall's body precursors and faint immunoreactivity for T-lymphocytes (CD3-positive) were detected from gd60 onwards. First macrophages (CD68 positive) were apparent at day 70, first CD20 immunoreactive cells (B-lymphocyte-like cells) at gd85, and natural killer cells (M1014 immunoreactive) at gd100. At gd100 all evaluated cell populations present in the adult cynomolgus monkey thymus were in place, whereas no B- and T-cell precursors or (CD34 and CD117, respectively) dendritic cells (CD35 positive cells) were present. All these immunopositive cells persisted, partly with changing distribution patterns, until the advanced age of 21years with the exception of natural killer cells, which were present only until adult ages (evaluation at 4-7years). The rationale of this study was to analyse thymic development in the cynomolgus monkey and to evaluate the relevance of the development of thymus in non-human primate as a model for corresponding human targeted toxicological research. Springer Science+Business Media, Inc., 2006 Non-human primate nationallicence Immune system nationallicence Thymus development nationallicence Toxicology nationallicence Buse Eberhard Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut Habermann Gunnar Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut Vogel Friedhelm Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 161-170 1567-2379 37:3-4<161 2006 37 10735 https://doi.org/10.1007/s10735-006-9057-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9057-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buse Eberhard Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut NATIONALLICENCE 700 1- Habermann Gunnar Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut NATIONALLICENCE 700 1- Vogel Friedhelm Covance Laboratories GmbH, Kesselfeld 29, 48163, Muenster, Germany aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 161-170 1567-2379 37:3-4<161 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer