caa a22 4500 467905533 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20060501xx s 000 0 eng 10.1007/s10735-006-9016-y doi (NATIONALLICENCE)springer-10.1007/s10735-006-9016-y Myenteric denervation differentially reduces enteroendocrine serotonin cell population in rats during postnatal development [Elektronische Daten] [Luzmarina Hernandes, Marilda da Cruz Fernandes, Lucieni Cristina Marques da Silva Pereira, Priscila de Freitas, Patrícia Gama, Eliana Parisi Alvares] Summary: The enteric nervous and enteroendocrine systems regulate different processes in the small intestine. Ablation of myenteric plexus with benzalkonium chloride (BAC) stimulates epithelial cell proliferation, whereas endocrine serotonin cells may inhibit the process. To evaluate the connection between the systems and the influence of myenteric plexus on serotoninergic cells in rats during postnatal development, the ileal plexus was partially removed with BAC. Rats were treated at 13 or 21days and sacrificed after 15days. The cell bodies of myenteric neurons were stained by β NADH-diaphorase to detect the extension of denervation. The number of enteroendocrine cells in the ileum was estimated in crypts and villi in paraffin sections immunostained for serotonin. The number of neurons was reduced by 27.6 and 45% in rats treated on the 13th and 21st days, respectively. We tried to establish a correlation of denervation and the serotonin population according to the age of treatment. We observed a reduction of immunolabelled cells in the crypts of rats treated at 13days, whereas this effect was seen in the villi of rats denervated at 21days. These results suggest that the enteric nervous system might control the enteroendocrine cell population and this complex mechanism could be correlated to changes in cell proliferation. Springer Science+Business Media, Inc., 2006 Hernandes Luzmarina Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut Fernandes Marilda da Cruz Department of Morphological Sciences, Faculty of Medical Sciences of Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil aut Pereira Lucieni Cristina Marques da Silva Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut Freitas Priscila de Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut Gama Patrícia Department of Cell and Developmental Biology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil aut Alvares Eliana Parisi Department of Morphological Sciences, Faculty of Medical Sciences of Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil aut Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 95-100 1567-2379 37:3-4<95 2006 37 10735 https://doi.org/10.1007/s10735-006-9016-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9016-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hernandes Luzmarina Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut NATIONALLICENCE 700 1- Fernandes Marilda da Cruz Department of Morphological Sciences, Faculty of Medical Sciences of Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Pereira Lucieni Cristina Marques da Silva Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut NATIONALLICENCE 700 1- Freitas Priscila de Morphophysiologycal Sciences Department, Universidade Estadual de Maringá, Avenida Colombo, 5790, CEP 87020-900, Maringá, Paraná, Brazil aut NATIONALLICENCE 700 1- Gama Patrícia Department of Cell and Developmental Biology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil aut NATIONALLICENCE 700 1- Alvares Eliana Parisi Department of Morphological Sciences, Faculty of Medical Sciences of Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/3-4(2006-05-01), 95-100 1567-2379 37:3-4<95 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer