caa a22 4500 46790569X CHVBK 20180406152833.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10735-006-9065-2 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9065-2 Ultrastructural morphometric analysis of ameloblasts exposed to fluoride during tooth development [Elektronische Daten] [Daniel Ribeiro, Luciane Hirota, Tania Cestari, Daniele Ceolin, Rumio Taga, Gerson Assis] Since a considerable amount of the world population is exposed to high doses of fluoride, it is of special concern to investigate its action mechanisms during dental enamel development. In this study, the toxicity of fluoride in ameloblasts during enamel development was evaluated by means of ultrastructural morphometric analysis. A total of 18 male Wistar rats were distributed into three groups. In Group I, the animals received deionized drinking water ad libitum (negative control) and in Groups II and III, they received sodium fluorided (NaF) drinking water at doses of 7 and 100ppm ad libitum, respectively, for 6weeks. Morphometric data were expressed as volume density of the most significant organelles present in the secretory and maturation phases of amelogenesis such as RER, granules, lysosomes, phagic vacuoles, microfilaments and mitochondria. The results showed that the volume density of mitochondria in the 100ppm experimental group was 29% (P<0.05) higher than the control group in secretory ameloblasts. No remarkable differences were found in maturation ameloblasts for all organelles evaluated. Taken together, these data indicate that NaF at high doses is able to induce cellular damage in secretory ameloblasts, whereas no noxious effect was observed during maturation stage of amelogenesis as depicted by ultrastructural analysis. Springer Science+Business Media, Inc., 2006 Dental fluorosis nationallicence Fluoride nationallicence Ameloblast nationallicence Rat incisor nationallicence Ultrastructure nationallicence Ribeiro Daniel Department of Health Sciences, Federal University of Sao Paulo, UNIFESP, Av. Ana Costa 95, 110601-001, Santos, SP, Brazil aut Hirota Luciane Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut Cestari Tania Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut Ceolin Daniele Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut Taga Rumio Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut Assis Gerson Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 361-367 1567-2379 37:8-9<361 2006 37 10735 https://doi.org/10.1007/s10735-006-9065-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9065-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ribeiro Daniel Department of Health Sciences, Federal University of Sao Paulo, UNIFESP, Av. Ana Costa 95, 110601-001, Santos, SP, Brazil aut NATIONALLICENCE 700 1- Hirota Luciane Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Cestari Tania Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Ceolin Daniele Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Taga Rumio Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Assis Gerson Department of Biological Sciences, Laboratory of Histology, Bauru School of Dentistry, USP, Sao Paulo, SP, Brazil aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 361-367 1567-2379 37:8-9<361 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer