caa a22 4500 467905703 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10735-006-9060-7 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9060-7 Expression of genes for gelatinases and tissue inhibitors of metalloproteinases in periodontal tissues during orthodontic tooth movement [Elektronische Daten] [Ichiro Takahashi, Kazuyuki Onodera, Makoto Nishimura, Hidetoshi Mitnai, Yasuyuki Sasano, Hideo Mitani] Orthodontic tooth movement progresses by a combination of periodontal ligament (PDL) tissue and alveolar bone remodeling processes. Besides the remodeling of alveolar bone around the moving teeth, the major extracellular matrix (ECM) components of PDLs, collagens, are degenerated, degraded, and restructured. Matrix metalloproteinases (MMPs) and their specific inhibitors, tissue inhibitors of metalloproteinases (TIMPs), act in a co-ordinated fashion to regulate the remodeling of periodontal tissues. We hypothesized that the expression levels of the genes for MMP-2, MMP-9, and TIMPs 1-3 are increased transiently in the periodontal tissue during orthodontic tooth movement. To test this hypothesis, we employed an animal model of tooth movement using rats, as well as in situ hybridization to analyze the expression levels of Mmp-2, Mmp-9, and Timps 1-3. The expression levels of these genes increased transiently in cells of periodontal tissues, which include cementoblasts, fibroblasts, osteoblasts, and osteoclasts, at the compression side of the moving teeth. The transient increases in gene expression at the tension side were mainly limited to osteoblasts and cementoblasts. In conclusion, the expression levels of Mmp-2, Mmp-9, and Timps 1-3 increase transiently during orthodontic tooth movement at both the tension and compression sides. The expression of these genes is regulated differentially in the periodontal tissue of the tension side and compression side. This altered pattern of gene expression may determine the rate and extent of remodeling of the collagenous ECM in periodontal tissues during orthodontic tooth movement. Springer Science+Business Media, Inc., 2006 Tooth movement nationallicence MMP nationallicence TIMP nationallicence Gene expression nationallicence Periodontal tissue remodeling nationallicence In situ hybridization nationallicence Takahashi Ichiro Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Onodera Kazuyuki Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Nishimura Makoto Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Mitnai Hidetoshi Division of Oral Dysfunction Science, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Sasano Yasuyuki Division of Craniofacial Development and Regeneration, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Mitani Hideo Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 333-342 1567-2379 37:8-9<333 2006 37 10735 https://doi.org/10.1007/s10735-006-9060-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9060-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Ichiro Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Onodera Kazuyuki Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Nishimura Makoto Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Mitnai Hidetoshi Division of Oral Dysfunction Science, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Sasano Yasuyuki Division of Craniofacial Development and Regeneration, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Mitani Hideo Division of Orthodontics and Dentofacial Orthopedics, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 333-342 1567-2379 37:8-9<333 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer