caa a22 4500 46790572X CHVBK 20180406152833.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10735-006-9067-0 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9067-0 EphB3 receptor and ligand expression in the adult rat brain [Elektronische Daten] [Christopher Willson, Roy Foster, Stephen Onifer, Scott Whittemore, Jorge Miranda] Summary: Eph receptors and ligands are two families of proteins that control axonal guidance during development. Their expression was originally thought to be developmentally regulated but recent work has shown that several EphA receptors are expressed postnatally. The EphB3 receptors are expressed during embryonic development in multiple regions of the central nervous system but their potential expression and functional role in the adult brain is unknown. We used in situ hybridization, immunohistochemistry, and receptor affinity probe in situ staining to investigate EphB3 receptors mRNA, protein, and ligand (ephrin-B) expression, respectively, in the adult rat brain. Our results indicate that EphB3 receptor mRNA and protein are constitutively expressed in discrete regions of the adult rat brain including the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices. The spatial profile of EphB3 receptors was co-localized to regions of the brain that had a high level of EphB3 receptor binding ligands. Its expression pattern suggests that EphB3 may play a role in the maintenance of mature neuronal connections or re-arrangement of synaptic connections during late stages of development. Springer Science+Business Media, Inc., 2006 EphB3 nationallicence Ephrin nationallicence Immunohistochemistry nationallicence In situ hybridization nationallicence Plasticity nationallicence Willson Christopher Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut Foster Roy Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut Onifer Stephen Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut Whittemore Scott Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut Miranda Jorge Department of Physiology, University of Puerto Rico School of Medicine, PO Box 365067, 00936-5067, San Juan, PR, USA aut Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 369-380 1567-2379 37:8-9<369 2006 37 10735 https://doi.org/10.1007/s10735-006-9067-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9067-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Willson Christopher Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 700 1- Foster Roy Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 700 1- Onifer Stephen Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 700 1- Whittemore Scott Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA aut NATIONALLICENCE 700 1- Miranda Jorge Department of Physiology, University of Puerto Rico School of Medicine, PO Box 365067, 00936-5067, San Juan, PR, USA aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 369-380 1567-2379 37:8-9<369 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer