caa a22 4500 467905738 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20061101xx s 000 0 eng 10.1007/s10735-006-9056-3 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9056-3 Langerhans cells in cutaneous tumours: immunohistochemistry study using a computer image analysis system [Elektronische Daten] [Mario De Melo Jr, Jorge Filho, Vasco Patu, Marcos Machado, Luciano Mello, Luiz Carvalho Jr] Immunohistochemistry, based on antibody anti-S100 protein, was used to evaluate the Langerhans cells (LC) in benign and malign skin neoplasias. These cells were quantitatively estimated using a computer image analysis (OPTIMAS software system, Version 6.1) in skin biopsies diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborreic keratosis (SK) and actinic keratosis (AK). The antibody anti-S100 protein recognized them. No significant variations were observed in the number of LC among malignant tumour (BCC=23.25±5.81 and EpC=20.88±4.24). Benign lesions (AK=33.04±7.11; TE=55.74±9.35; SK=42.38±9.92, and KA=47.62±10.4) presented a higher number of LC when they were compared among them and to malignant and normal tissues. No significant differences were observed in LC area and volume between benign and malign neoplasias. These results indicate possibly differences in the immunogenicity between benign and malign epidermic tumours. In conclusion, the experimental computer assessment method was reliable and consistent to morphometric analysis of tumoural tissues. Springer Science+Business Media, Inc., 2006 Langerhans cell nationallicence S-100 protein nationallicence Neoplasias nationallicence Image analysis programme nationallicence De Melo Jr Mario Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Filho Jorge Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Patu Vasco Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Machado Marcos Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Mello Luciano Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Carvalho Jr Luiz Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 321-325 1567-2379 37:8-9<321 2006 37 10735 https://doi.org/10.1007/s10735-006-9056-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9056-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Melo Jr Mario Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Filho Jorge Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Patu Vasco Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Machado Marcos Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Mello Luciano Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 700 1- Carvalho Jr Luiz Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910, Recife, PE, Brazil aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/8-9(2006-11-01), 321-325 1567-2379 37:8-9<321 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer