caa a22 4500 467905762 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10735-006-9026-9 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9026-9 Immunogold study of altered expression of some interendothelial junctional molecules in the brain blood microvessels of diabetic scrapie-infected mice [Elektronische Daten] [Andrzej Vorbrodt, Danuta Dobrogowska, Michal Tarnawski, Harry Meeker, Richard Carp] Quantitative immunogold procedure was used to study the distribution of molecular components of interendothelial junctions in blood-brain barrier (BBB) microvessels of scrapie infected SJL/J hyperglycemic mice showing obesity and reduced glucose tolerance. Samples of brain (fronto-parietal cerebral cortex and thalamo-hypothalamic region) obtained from hyperglycemic (diabetic) mice and from non- infected, normoglycemic (non-diabetic) SJL/J mice, were processed for immunocytochemical examination. The localization of the following tight junction (TJ)-associated proteins was studied: occludin as an integral membrane (transmembrane) protein, and zonula occludens one (ZO-1) as a peripheral protein. The localization of β-catenin as a representative of the cadherin/catenin complex that is typical for adherens junctions (AJs) also was studied. Morphometric analysis revealed that the density of immunosignals for occludin, represented by colloidal gold particles (GPs), was significantly lower in the brain microvessels of diabetic than in non-diabetic mice. No significant differences in the density of immunosignals for ZO-1 and β-catenin between both experimental mouse groups were observed. It indicates that abnormal glucose metabolism affects mostly occludin which is believed to play a fundamental role in the maintenance of the tightness of endothelial lining in brain microvascular network and thereby in the preservation of its barrier function. These results also support the previously expressed opinion that occludin, detected with the applied morphological method, can be considered a sensitive indicator of altered molecular architecture of the interendothelial junctions due to the action of some metabolic or pathological insults. Springer Science+Business Media B.V., 2006 Interendothelial junctions nationallicence Blood-brain barrier nationallicence Subcellular localization of junctional molecules nationallicence Immunogold cytochemistry nationallicence Vorbrodt Andrzej Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut Dobrogowska Danuta Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut Tarnawski Michal Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut Meeker Harry Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut Carp Richard Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 27-35 1567-2379 37:1-2<27 2006 37 10735 https://doi.org/10.1007/s10735-006-9026-9 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9026-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vorbrodt Andrzej Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Dobrogowska Danuta Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Tarnawski Michal Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Meeker Harry Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Carp Richard Laboratory of Cytochemistry, Department of Developmental Neurobiology, New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, 10314, Staten Island, NY, USA aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 27-35 1567-2379 37:1-2<27 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer