caa a22 4500 467905770 CHVBK 20180406152833.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10735-006-9025-x doi (NATIONALLICENCE)springer-10.1007/s10735-006-9025-x Detection of guinea pig macrophages by a new CD68 monoclonal antibody, PM-1K [Elektronische Daten] [Tomohiro Horikawa, Yoshihiro Komohara, Emi Kiyota, Yasuhiro Terasaki, Katsumasa Takagi, Motohiro Takeya] A new monoclonal antibody, PM-1K, was raised against 24-h cultured human peritoneal macrophages. In immunohistochemical assays, PM-1K recognized freshly isolated blood monocytes and most tissue macrophages as well as myeloid dendritic cells such as Langerhans cells and interdigitating cells. The molecular size of the antigen recognized by PM-1K was determined to be 110kD by means of immunoaffinity purification. Because this affinity-purified antigen recognized by PM-1K was also recognized by anti-CD68 antibodies, it is believed to be one of the heterogeneous molecules of the CD68 antigen. Analysis showed interspecies reactivity of PM-1K with macrophages from guinea pigs, pigs, bovine species, and monkeys. Among these macrophages, those of the guinea pig reacted strongly with PM-1K. Patterns of PM-1K immunostaining in guinea pig tissues were similar to those found in human tissues. Studies with the immunoelectron microscope revealed reaction products of PM-1K in the cytoplasm, especially around endosomes. Since only a few antibodies are available to label guinea pig macrophages, PM-1K is considered to be one of the most suitable antibodies to examine macrophages in experimental guinea pig models. Springer Science+Business Media B.V., 2006 CD68 nationallicence Macrophages nationallicence Guinea pigs nationallicence Immunohistochemistry nationallicence Monoclonal antibody nationallicence Horikawa Tomohiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Komohara Yoshihiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Kiyota Emi Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Terasaki Yasuhiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Takagi Katsumasa Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Takeya Motohiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 15-25 1567-2379 37:1-2<15 2006 37 10735 https://doi.org/10.1007/s10735-006-9025-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9025-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Horikawa Tomohiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 700 1- Komohara Yoshihiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 700 1- Kiyota Emi Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 700 1- Terasaki Yasuhiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 700 1- Takagi Katsumasa Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 700 1- Takeya Motohiro Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 15-25 1567-2379 37:1-2<15 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer