caa a22 4500 467905827 CHVBK 20180406152834.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10735-006-9031-z doi (NATIONALLICENCE)springer-10.1007/s10735-006-9031-z Distinct immunohistochemical expression of osteopontin in the adult rat major salivary glands [Elektronische Daten] [Nicholas Obermüller, Nikolaus Gassler, Norbert Gretz, Bettina Kränzlin, Sigrid Hoffmann, Helmut Geiger, Stefan Gauer] Osteopontin is a multifunctional protein secreted by epithelial cells of various tissues. Its expression in the adult rat major salivary glands has not yet been studied. We examined osteopontin expression by immunohistochemistry using a well characterized monoclonal antibody. Submandibular glands of young adult male rats (70-100days old) showed specific expression in secretion granules of granular duct cells but also in cells of the striated ducts and excretory duct. In the major sublingual as well as the parotid gland expression was found solely in the duct system. In addition, a few interstitial-like cells exhibiting very strong immunostaining for osteopontin could be found in either organ. Expression could neither be seen in acinar cells nor in cells of the intercalated ducts. Moreover, in submandibular glands of more aged rats (6- to 7-month old) which show well developed granular convoluted tubules, there was almost exclusive expression of osteopontin in granular duct cells as well as in some interstitial-like cells, but barely in the striated/excretory duct system. Western blot analysis of the submandibular gland showed a specific band migrating at approximately 74kDa, detectable at both age stages. Osteopontin secreted fom granular duct cells may influence the compostion of the saliva, e.g. thereby modulating pathways affecting sialolithiasis. Its expression in striated duct cells may also hint to roles such as cell-cell attachment or cell differentiation. The cell-specific expression detected in the rat major salivary glands differs in part from that reported in mice, human and monkey. Springer Science+Business Media B.V., 2006 Salivary glands nationallicence Osteopontin nationallicence Rat nationallicence Obermüller Nicholas Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut Gassler Nikolaus Institute of Pathology, University Hospital, RWTH Aachen, Aachen, Germany aut Gretz Norbert Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut Kränzlin Bettina Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut Hoffmann Sigrid Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut Geiger Helmut Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut Gauer Stefan Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 53-60 1567-2379 37:1-2<53 2006 37 10735 https://doi.org/10.1007/s10735-006-9031-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9031-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Obermüller Nicholas Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut NATIONALLICENCE 700 1- Gassler Nikolaus Institute of Pathology, University Hospital, RWTH Aachen, Aachen, Germany aut NATIONALLICENCE 700 1- Gretz Norbert Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut NATIONALLICENCE 700 1- Kränzlin Bettina Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut NATIONALLICENCE 700 1- Hoffmann Sigrid Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany aut NATIONALLICENCE 700 1- Geiger Helmut Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut NATIONALLICENCE 700 1- Gauer Stefan Division of Nephrology, Medical Clinic III, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt/M, Germany aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 53-60 1567-2379 37:1-2<53 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer