caa a22 4500 467905835 CHVBK 20180406152834.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10735-006-9037-6 doi (NATIONALLICENCE)springer-10.1007/s10735-006-9037-6 The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography [Elektronische Daten] [Ichiro Sakuraba, Junko Hatakeyama, Yuji Hatakeyama, Ichiro Takahashi, Hideaki Mayanagi, Yasuyuki Sasano] Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation. Springer Science+Business Media B.V., 2006 Tooth nationallicence Matrix metalloproteinases nationallicence Extracellular matrix nationallicence In situ zymography nationallicence Dental root development nationallicence Enzyme nationallicence Sakuraba Ichiro Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut Hatakeyama Junko Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan aut Hatakeyama Yuji Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut Takahashi Ichiro Division of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut Mayanagi Hideaki Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut Sasano Yasuyuki Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan aut Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 87-93 1567-2379 37:1-2<87 2006 37 10735 https://doi.org/10.1007/s10735-006-9037-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-006-9037-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakuraba Ichiro Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Hatakeyama Junko Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan aut NATIONALLICENCE 700 1- Hatakeyama Yuji Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Takahashi Ichiro Division of Orthodontic and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Mayanagi Hideaki Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Sasano Yasuyuki Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 87-93 1567-2379 37:1-2<87 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer