caa a22 4500 467905851 CHVBK 20180406152834.0 cr unu---uuuuu 170328e20060101xx s 000 0 eng 10.1007/s10735-005-9011-8 doi (NATIONALLICENCE)springer-10.1007/s10735-005-9011-8 Immunofluorescent Identification of Melanocytes in Murine Hair Follicles [Elektronische Daten] [Dheshnie Govender, Lester Davids, Susan Kidson] Summary: Immunocytochemical identification of skin cells are difficult due to numerous endogenous autofluorescent components within the cell and the environment. This is particularly evident in hair follicles. This paper reports on a serendipitous modification to an existing method which results in a drastically reduced background fluorescence. Immediately after antigen retrieval, sections exposed to 0.3% hydrogen peroxide in methanol for 30min at room temperature exhibited low background fluorescence, increased antigenicity and revealed quantifiable numbers of melanocytes. This method is applicable to both human and mouse melanocytes particularly in the hair follicle. Springer Science+Business Media, Inc., 2006 flourescent microscopy nationallicence hydrogen peroxide nationallicence melanocytes nationallicence murine hair follicles nationallicence Govender Dheshnie Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut Davids Lester Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut Kidson Susan Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 1-3 1567-2379 37:1-2<1 2006 37 10735 https://doi.org/10.1007/s10735-005-9011-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10735-005-9011-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Govender Dheshnie Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut NATIONALLICENCE 700 1- Davids Lester Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut NATIONALLICENCE 700 1- Kidson Susan Department of Human Biology, Anatomy Building, Faculty of Health Sciences, University of Cape Town Medical School, 7925 Cape Town, Observatory, South Africa aut NATIONALLICENCE 773 0- Journal of Molecular Histology Kluwer Academic Publishers 37/1-2(2006-01-01), 1-3 1567-2379 37:1-2<1 2006 37 10735 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer