caa a22 4500 467906777 CHVBK 20180406152836.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00251-005-0070-5 doi (NATIONALLICENCE)springer-10.1007/s00251-005-0070-5 Identification and regulatory analysis of rainbow trout tapasin and tapasin-related genes [Elektronische Daten] [Eric Landis, Yniv Palti, Jenefer Dekoning, Robert Drew, Ruth Phillips, John Hansen] Tapasin (TAPBP) is a key member of MHC class Ia antigen-loading complexes, bridging the class Ia molecule to the transporter associated with antigen presentation (TAP). As part of an ongoing study of MHC genomics in rainbow trout, we have identified two rainbow trout TAPBP genes (Onmy-TAPBP.a and .b) and a similar but distinct TAPBP-related gene (Onmy-TAPBP-R) that had previously only been described in mammals. Physical and genetic mapping indicate that Onmy-TAPBP.a is on chromosome 18 in the MHC class Ia region and that Onmy-TAPBP.b resides on chromosome 14 in the MHC class Ib region. There are also at least two copies of TAPBP-R, Onmy-TAPBP-R.a and Onmy-TAPBP-R.b, located on chromosomes 2 and 3, respectively. Due to the central role of TAPBP expression during acute viral infection, we have characterized the transcriptional profile and regulatory regions for both Onmy-TAPBP and Onmy-TAPBP-R. Transcription of both genes increased during acute infection with infectious hematapoeitic necrosis virus (IHNV) in a fashion indicative of interferon-mediated regulation. Promoter-reporter assays in STE-137 cells demonstrate that the trout TAPBP and TAPBP-R promoters respond to interferon regulatory factors, Onmy-IRF1 and Onmy-IRF2. Overall, TAPBP is expressed at higher levels than TAPBP-R in naïve tissues and TAPBP transcription is more responsive to viral infection and IRF1 and 2 binding. Springer-Verlag, 2006 Tapasin nationallicence Antigen presentation/processing nationallicence Gene regulation nationallicence MHC nationallicence Landis Eric Molecular Medicine Program, University of Maryland Medical School, Baltimore, Maryland, USA aut Palti Yniv USDA-ARS-NCCCWA, 11876 Leetown Road, WV 25430, Kearneysville, USA aut Dekoning Jenefer Department of Biological Sciences, Washington State University, Vancouver, Washington, USA aut Drew Robert School of Biological Sciences, Washington State University, Pullman, Washington, USA aut Phillips Ruth Department of Biological Sciences, Washington State University, Vancouver, Washington, USA aut Hansen John Molecular Medicine Program, University of Maryland Medical School, Baltimore, Maryland, USA aut Immunogenetics Springer-Verlag 58/1(2006-02-01), 56-69 0093-7711 58:1<56 2006 58 251 https://doi.org/10.1007/s00251-005-0070-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0070-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Landis Eric Molecular Medicine Program, University of Maryland Medical School, Baltimore, Maryland, USA aut NATIONALLICENCE 700 1- Palti Yniv USDA-ARS-NCCCWA, 11876 Leetown Road, WV 25430, Kearneysville, USA aut NATIONALLICENCE 700 1- Dekoning Jenefer Department of Biological Sciences, Washington State University, Vancouver, Washington, USA aut NATIONALLICENCE 700 1- Drew Robert School of Biological Sciences, Washington State University, Pullman, Washington, USA aut NATIONALLICENCE 700 1- Phillips Ruth Department of Biological Sciences, Washington State University, Vancouver, Washington, USA aut NATIONALLICENCE 700 1- Hansen John Molecular Medicine Program, University of Maryland Medical School, Baltimore, Maryland, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/1(2006-02-01), 56-69 0093-7711 58:1<56 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer