caa a22 4500 467906807 CHVBK 20180406152836.0 cr unu---uuuuu 170328e20060201xx s 000 0 eng 10.1007/s00251-005-0056-3 doi (NATIONALLICENCE)springer-10.1007/s00251-005-0056-3 Identification and characterization of two related murine genes, Eat2a and Eat2b , encoding single SH2-domain adapters [Elektronische Daten] [Silvia Calpe, Erika Erdős, Gongxian Liao, Ninghai Wang, Svend Rietdijk, Maria Simarro, Beata Scholtz, Jill Mooney, Chang Lee, Min Shin, Éva Rajnavölgyi, John Schatzle, Herbert Morse III, Cox Terhorst, Arpad Lanyi] Human EAT-2 (SH2D1B) and SLAM-associated protein (SAP) (SH2D1A) are single SH2-domain adapters, which bind to specific tyrosine residues in the cytoplasmic tail of six signaling lymphocytic activation molecule (SLAM) (SLAMF1)-related receptors. Here we report that, unlike in humans, the mouse and rat Eat2 genes are duplicated with an identical genomic organization. The coding regions of the mouse Eat2a and Eat2b genes share 91% identity at the nucleotide level and 84% at the protein level; similarly, segments of introns are highly conserved. Whereas expression of mouse Eat2a mRNA was detected in multiple tissues, Eat2b was only detectable in mouse natural killer cells, CD8+ T cells, and ovaries, suggesting a very restricted tissue expression of the latter. Both the EAT-2A and EAT-2B coimmunoprecipitated with mouse SLAM in transfected cells and augmented tyrosine phosphorylation of the cytoplasmic tail of SLAM. Both EAT-2A and EAT-2B bind to the Src-like kinases Fyn, Hck, Lyn, Lck, and Fgr, as determined by a yeast two-hybrid assay. However, unlike SAP, the EAT-2 proteins bind to their kinase domains and not to the SH3 domain of these kinases. Taken together, the data suggest that both EAT-2A and EAT-2B are adapters that recruit Src kinases to SLAM family receptors using a mechanism that is distinct from that of SAP. Springer-Verlag, 2006 Adapter proteins nationallicence EAT-2 nationallicence SLAM nationallicence Src kinases nationallicence Calpe Silvia Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Erdős Erika Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut Liao Gongxian Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Wang Ninghai Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Rietdijk Svend Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Simarro Maria Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Scholtz Beata Department of Biochemistry and Molecular Biology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut Mooney Jill Department of Pathology and Center for Immunology, University of Texas Southwestern Medical Center, 75390, Dallas, TX, USA aut Lee Chang Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut Shin Min Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut Rajnavölgyi Éva Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut Schatzle John Department of Pathology and Center for Immunology, University of Texas Southwestern Medical Center, 75390, Dallas, TX, USA aut Morse III Herbert Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut Terhorst Cox Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut Lanyi Arpad Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut Immunogenetics Springer-Verlag 58/1(2006-02-01), 15-25 0093-7711 58:1<15 2006 58 251 https://doi.org/10.1007/s00251-005-0056-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-005-0056-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Calpe Silvia Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Erdős Erika Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut NATIONALLICENCE 700 1- Liao Gongxian Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Wang Ninghai Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Rietdijk Svend Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Simarro Maria Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Scholtz Beata Department of Biochemistry and Molecular Biology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut NATIONALLICENCE 700 1- Mooney Jill Department of Pathology and Center for Immunology, University of Texas Southwestern Medical Center, 75390, Dallas, TX, USA aut NATIONALLICENCE 700 1- Lee Chang Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut NATIONALLICENCE 700 1- Shin Min Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut NATIONALLICENCE 700 1- Rajnavölgyi Éva Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut NATIONALLICENCE 700 1- Schatzle John Department of Pathology and Center for Immunology, University of Texas Southwestern Medical Center, 75390, Dallas, TX, USA aut NATIONALLICENCE 700 1- Morse III Herbert Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, 5640 Fishers Lane, 20852, Rockville, MD, USA aut NATIONALLICENCE 700 1- Terhorst Cox Division of Immunology BIDMC, Harvard Medical School, 77 Ave. Louis Pasteur, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Lanyi Arpad Institute of Immunology, University of Debrecen Medical and Health Science Center, 98 Nagyerdei Blv., 4012, Debrecen, Hungary aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/1(2006-02-01), 15-25 0093-7711 58:1<15 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 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