caa a22 4500 46790684X CHVBK 20180406152836.0 cr unu---uuuuu 170328e20060901xx s 000 0 eng 10.1007/s00251-006-0136-z doi (NATIONALLICENCE)springer-10.1007/s00251-006-0136-z Identification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells [Elektronische Daten] [Carolyn Herzig, Seth Blumerman, Cynthia Baldwin] To understand the biology of γδ T cells in ruminants, it is necessary to have a comprehensive picture of γδ T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor δ (TRD) variable genes were identified by RT-PCR and sequencing and homology with TRDV genes from other mammals determined. Previously unidentified TRDV subgroup genes described in this study include the bovine homologues of ovine TRDV2, TRDV3, and TRDV4 which were named accordingly. TRDV2 subgroup has two genes (TRDV2-1 and TRDV2-2) while we found the previously identified TRDV1 has at least eight genes corresponding to separate genomic sequences. Nucleotide and amino acid sequences for particular gene subgroups between cattle and sheep were more than 87% identical but identities among TRDV subgroups within a species were much less, with bovine TRDV4 having <45% identity to the other three bovine TRDV gene subgroups. Analysis of circulating bovine γδ T cells revealed that genes from all four TRDV subgroups were expressed in combination with TRDJ1, TRDJ3, and TRDC, although TRDV4 was the least represented, and all displayed a variety of CDR3 junctional lengths. Finally, some genes within the TRDV1, TRDV2, and TRDV3 subgroups recombined with TRAV incorporating TRAJs, suggesting dual use. Springer-Verlag, 2006 γδ T cells nationallicence γδ T-cell receptor nationallicence γδ T-cell receptor genes nationallicence Ruminant nationallicence TRDV nationallicence TCR δ nationallicence Herzig Carolyn Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut Blumerman Seth Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut Baldwin Cynthia Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut Immunogenetics Springer-Verlag 58/9(2006-09-01), 746-757 0093-7711 58:9<746 2006 58 251 https://doi.org/10.1007/s00251-006-0136-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00251-006-0136-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herzig Carolyn Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Blumerman Seth Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 700 1- Baldwin Cynthia Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, 01003, Amherst, MA, USA aut NATIONALLICENCE 773 0- Immunogenetics Springer-Verlag 58/9(2006-09-01), 746-757 0093-7711 58:9<746 2006 58 251 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer